G Protein-Coupled Receptor 12 antibody (Extracellular)

Catalog No. ABIN7581884

The Rabbit Polyclonal anti-G Protein-Coupled Receptor 12 antibody has been validated for WB and IHC. It is suitable to detect G Protein-Coupled Receptor 12 in samples from Mouse.

Quick Overview for G Protein-Coupled Receptor 12 antibody (Extracellular) (ABIN7581884)

Target: G Protein-Coupled Receptor 12 (GPR12)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This G Protein-Coupled Receptor 12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-G Protein-Coupled Receptor 12 Antibody

Binding Specificity: AA 3-15, Extracellular

Purpose: A Rabbit Polyclonal antibody to SORCS2

Sequence: (C)EDPKVNLSGL PRD

Specificity: Extracellular, N-term.

Predicted Reactivity: Rat - identical,Human - 12 out of 13 amino acid residues identical

Characteristics: Anti-GPR12 (extracellular) Antibody (ABIN7581884) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR12 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EDPKVNLSGLPRD, corresponding to amino acid residues 3 - 15 of mouse GPR12 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for G Protein-Coupled Receptor 12

Target: G Protein-Coupled Receptor 12 (GPR12)

Alternative Name: GPR12 

Background: G-Protein Coupled Receptor 12, GPCR21,G-protein-coupled receptors (GPCRs), are the largest family of membrane proteins and mediate most of the physiological responses to hormones, neurotransmitters and environmental stimulants. At the most basic level, all GPCRs are characterized by the presence of seven membrane-spanning α-helical segments separated by alternating intracellular and extracellular loop regions1. Orphan GPCRs is a group of GPCRs that their endogenous ligands remain unidentified and therefore their natural functions in doubt2.GPR12 is an orphan G protein-coupled receptor related phylogenetically, functionally, and pharmacologically with the cannabinoid system. It considered as a "constitutively active receptor" since it greatly stimulates adenylate cyclase in the absence of ligand-receptor interaction. GPR12 seems to couple Gαs as well as Gαi proteins since it stimulates baseline cAMP-dependent signaling by activating Gαs proteins, and also inhibits forskolin activated signaling through Gαi interaction3.GPR12 expressed in the pituitary gland, in neurons in the frontal cortex, piriform cortex, thalamus, hypothalamus, hippocampus, amygdala, and olfactory bulb. It was found that GPR12 plays a significant role in energy balance. GPR12 knock out mice exhibit increase body weight and body fat mass. In addition, overexpression of GPR12 leads to cancer cells migration and invasion via phosphorylation of Keratin 84.Furthermore, GPR12 has been identified as an inverse agonist of the phytocannabinoid cannabidiol (CBD)4.

Gene ID: 14738

UniProt: P35412