GPR160 antibody (Intracellular)

Catalog No. ABIN7581887

The Rabbit Polyclonal anti-GPR160 antibody has been validated for WB and IHC. It is suitable to detect GPR160 in samples from Rat.

Quick Overview for GPR160 antibody (Intracellular) (ABIN7581887)

Target: GPR160 (G Protein-Coupled Receptor 160 (GPR160))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR160 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GPR160 Antibody

Binding Specificity: AA 122-136, Intracellular

Purpose: A Rabbit Polyclonal antibody to GPR160

Sequence: CNLSRATKPS SRWQK

Specificity: Intracellular, 2nd loop.

Predicted Reactivity: Mouse - 13 out of 15 amino acid residues identical It won't recognize human samples

Characteristics: Anti-GPR160 Antibody (ABIN7581887) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize GPR160 from rat and mouse samples. The antibody will not recognize human GPR160.

Purification: Affinity purified on immobilized antigen.

Immunogen: CNLSRATKPSSRWQK, corresponding to amino acid residues 122 - 136 of rat GPR160

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR160

Target: GPR160 (G Protein-Coupled Receptor 160 (GPR160))

Alternative Name: GPR160

Background: G-Protein Coupled Receptor 160,G-protein coupled receptor 160, GP160 or GPR160, is an orphan class A GPCR that plays a role in pain signaling.1,3GPR160 is composed of 338 amino acids, encoded by 7 exons located at 3q26.2-q27, demonstrates a significant degree of conservation across various species, and is expressed in neurons, astrocytes, and microglia within the central nervous system (CNS) and spinal cord.3Cocaine- and amphetamine-regulated transcript peptide (CARTp) exhibits various pharmacological actions in the CNS, peripheral nervous system, and several endocrine organs. It plays a role in reward and addiction, anxiety and depression, and food intake and body weight maintenance. It has been found that the interaction between CARTp and GPR160 is essential for the circadian regulation of appetite and thirst as well as the transmission of pain signals induced by nerve injury. GPR160 has been shown to confer CARTp-induced cFOS and ERK phosphorylation and activation of CREB independently of cAMP signaling. ERK can function as an upstream regulator of CREB phosphorylation during the development of neuropathic pain. As a putative ligand for GPR160, endogenous CARTp inhibition has been shown to mitigate neuropathic pain.1,2GPR160 has also been shown to play a role in the pathogenesis of prostate cancer. Transcription profiles demonstrate a marked increase of GPR160 mRNA in all stages of prostate cancer samples and cancer cell lines, except prostate hyperplasia tissues, with levels comparable to those in stage II-IV cancer patients. GPR160 knockdown increased caspase 1 and IL-6 expression and induced cell cycle arrest and apoptosis. The involvement of GPR160 in prostate carcinogenicity has therapeutic implications for exploring diagnostics and pharmaceutical interventions.3

Gene ID: 499588

UniProt: Q66H29