NEGR1 antibody (Extracellular)

Catalog No. ABIN7581917

The Rabbit Polyclonal anti-NEGR1 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect NEGR1 in samples from Mouse.

Quick Overview for NEGR1 antibody (Extracellular) (ABIN7581917)

Target: NEGR1 (Neuronal Growth Regulator 1 (NEGR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEGR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NEGR1 Antibody

Binding Specificity: Extracellular

Purpose: A Rabbit polyclonal antibody to NEGR1 (extracellular).

Sequence: (C)HISPSAKPFE NGQYLD

Specificity: Extracellular. 

Predicted Reactivity: Human,Rat - identical 

Characteristics: Anti-NEGR1 (extracellular) Antibody (ABIN7581917) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NEGR1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)HISPSAKPFENGQYLD, corresponding to amino acid residues 168 -183 of mouse NEGR1

Isotype: IgG

Application Details

Application Notes: Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NEGR1

Target: NEGR1 (Neuronal Growth Regulator 1 (NEGR1))

Alternative Name: NEGR1

Background: Neuronal Growth Regulator 1, IgLON Family Member 4, IGLON4 ,Neuronal growth regulator 1 (NEGR1), also known as neurotractin or Kilon, is a member of the immunoglobulin superfamily's IgLON subgroup. Structurally, IgLON proteins share three Ig-like C2-type domains and are anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor1,2. These proteins are implicated in neural development, supporting cellular interactions, neurite outgrowth, and synaptic formation3,4. NEGR1 is predominantly expressed in brain tissue, especially in the cerebral cortex, hippocampus, and hypothalamus. At the cellular level, it localizes to neuronal cell membranes where it regulates neurite extension and synapse stabilization1,5. NEGR1 activity is regulated through interaction with signaling molecules such as leukemia inhibitory factor receptor (LIFR), which affects downstream factors like lipocalin-2 (Lcn2) that are vital for neurogenesis and affective behaviors3. Functionally, NEGR1 modulates cell adhesion and neurite growth, processes essential for the proper development of neural circuits. It plays roles in both physiological and pathological states, for example, it is linked to hippocampal neurogenesis and synaptic plasticity, influencing behaviors related to anxiety and depression3,5. Dysfunctions in NEGR1 are associated with obesity, learning difficulties, psychiatric disorders, and mood dysregulation. Knockout studies in mice reveal that the loss of NEGR1 leads to reduced body mass, anxiety-like behaviors, and impaired neurogenesis2,4.

Gene ID: 320840

Pathways: Feeding Behaviour