Nerve Growth Factor antibody

Catalog No. ABIN7581918

This anti-Nerve Growth Factor antibody is a Mouse Monoclonal antibody detecting Nerve Growth Factor in ELISA and Neut. Suitable for Mouse.

Quick Overview for Nerve Growth Factor antibody (ABIN7581918)

Target: Nerve Growth Factor (NGF)

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nerve Growth Factor antibody is un-conjugated

Application: ELISA, Neutralization (Neut)

Clone: HAL06

Product Details anti-Nerve Growth Factor Antibody

Purpose: A Mouse Monoclonal Antibody to NGF and proNGF Designed to Neutralize their Biological Functions

Sequence: Highly purified 2.5S mouse NGF

Cross-Reactivity (Details): The antibody has minimal cross-reactivity with BDNF, NT-3, and NT-4/5.

Predicted Reactivity:  NGF,proNGF from human,rat,mouse samples

Characteristics: Highly specific monoclonal antibody directed against an epitope of mouse NGF. Mouse NGF/proNGF Neutralizing Antibody (ABIN7581918) can be used in indirect ELISA and neutralization assays to block the biological effects of both NGF and proNGF. It was designed to recognize NGF and proNGF from human, rat and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: Highly purified 2.5S mouse NGF

Isotype: IgG1, IgG1 kappa

Application Details

Application Notes:

Antigen preadsorption control: NA

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: NA

Application Dilutions Western blot wb: NA

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL or 0.25 mL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Nerve Growth Factor

Target: Nerve Growth Factor (NGF)

Alternative Name: NGF

Background: Nerve growth factor,The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and the PNS.1 NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3 Neurotrophin secretion also increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induced cytokines combine to increase neurotrophin production in these cells after injury.4 NGF is purified in three forms: the 7S, 2.5S and β. The 7S, 130 kDa, form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The 2.5S form is 9 amino acids shorter than the β form, because of proteolysis that occurs during the purification process.6 The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as PDGF.7 There is a 95.8 % homology between the rat and mouse forms, and a 85 % homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.8 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,9 psoriasis10 and wound healing.11 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75, which binds all the neurotrophins.12

Gene ID: 18049

UniProt: P01139

Pathways: Regulation of Cell Size