NPBWR1 antibody (Intracellular)

Catalog No. ABIN7581920

The Rabbit Polyclonal anti-NPBWR1 antibody has been validated for WB and IHC. It is suitable to detect NPBWR1 in samples from Mouse.

Quick Overview for NPBWR1 antibody (Intracellular) (ABIN7581920)

Target: NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPBWR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NPBWR1 Antibody

Binding Specificity: AA 145-158, Intracellular

Purpose: A Rabbit Polyclonal antibody to NPBWR1 receptor

Sequence: (C)ESRRVSGRTY GAAR

Specificity: Intracellular, 2nd loop.

Cross-Reactivity (Details): The antibody is specific for NPBWR1, it will not recognize the human only receptor NPBWR2.

Predicted Reactivity: Rat - identical, human - 12 out of 14 amino acid residues identical

Characteristics: Anti-NPBWR1 Antibody (ABIN7581920) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPBWR1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ESRRVSGRTYGAAR, corresponding to amino acid residues 145 - 158 of mouse NPBWR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPBWR1

Target: NPBWR1 (Neuropeptides B/W Receptor 1 (NPBWR1))

Alternative Name: NPBWR1

Background: Neuropeptides B/W Receptor Type 1, G Protein-Coupled Receptor 7, GPR7,Neuropeptides B/W receptor type 1 (NPBWR1), also known as G-protein coupled receptor 7 (GPR7), is a member of the G-protein coupled receptor (GPCR) superfamily.NPBWR1 mediates the biological activities of Neuropeptide B (NPB) and neuropeptide W (NPW) that are structurally and functionally related endogenous neuropeptides. In humans, a second receptor termed NPBWR2 or GPR8, has been identified, however, NPBWR2 is absent in rodents.1,2NPBWR1 is expressed in the central nervous system (CNS) and in peripheral tissues. In the brain expression of NPBWR1 is found in several regions including hypothalamus, hippocampus and amygdala. Indeed, the distribution of NPBWR1 and its ligands in brain is in accordance to their established functions in appetite control and feeding behavior, as well as locomotion, analgesia and stress response.2,3Several studies have pointed to the role of NPBWR1 in pain modulation. Interestingly, NPBWR1 has structural similarities with the opioid receptors and in fact, it binds non-selective opioid ligands such as β-endorphin.3 It has been suggested, that a combination therapies of morphine (an opioid receptor ligand) and NPBWR1 agonists could provide a new avenue to treat inflammatory and neuropathic pain with less risk of adverse effects. 4In the periphery, NPBWR1 may be involved in energy homeostasis by regulating insulin secretion from pancreatic β cells and regulating white and brown adipose tissue. 3

Gene ID: 226304

UniProt: P49681