NPFFR1 antibody (Extracellular)

Catalog No. ABIN7581921

This anti-NPFFR1 antibody is a Rabbit Polyclonal antibody detecting NPFFR1 in WB and IHC. Suitable for Rat.

Quick Overview for NPFFR1 antibody (Extracellular) (ABIN7581921)

Target: NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPFFR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-NPFFR1 Antibody

Binding Specificity: AA 184-197, Extracellular

Purpose: A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 1 (extracellular)

Sequence: (C)REEHHFMLDA RNRS

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - identical Human - 13 out of 14 amino acid residues identical

Characteristics: Anti-Neuropeptide FF Receptor 1 (extracellular) Antibody (ABIN7581921) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize NPFFR1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)REEHHFMLDARNRS, corresponding to amino acid residues 184 - 197 of rat NPFFR1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPFFR1

Target: NPFFR1 (Neuropeptide FF Receptor 1 (NPFFR1))

Alternative Name: NPFFR1

Background: NPFFR1, G-Protein Coupled Receptor 147, GPR147, RFamide-Related Peptide Receptor OT7T022,Neuropeptide FF receptor 1, NPFFR1, also known as GPR147 and RFamide-related peptide receptor 0T7T022, is a Gi/o protein-coupled receptor that mainly couples to Gαi3 and Gαs proteins and binds NPFF and RF-amide related peptides that are characterized by a conserved C-terminal RF-amide motif.1,2NPFF has been identified in various species, including humans, rodents, bovines, and guinea pigs, indicating its physiological significance. NPFF has been detected in discrete areas of the rat central nervous system (CNS), with the highest levels observed in the dorsal spinal cord and the posterior lobe of the pituitary gland. The distribution of NPFF mRNA at the tissue level generally corresponds with NPFF immunoreactivity, except in the hypothalamic region where differences have been observed. The NPFF gene was found to be upregulated in the dorsal horn by inflammatory pain.1 NPFFR1 is expressed at a higher level than NPFFR2 in the human CNS, while NPFFR2 is more abundant than NPFFR1 in the rat brain. NPFFR1 mRNA is more abundant than NPFFR2 in the human spinal cord, whereas only NPFFR2 is found in the spinal cord of rats.1NPFFR1 displayed a sex-biased role in food intake and metabolic homeostasis. NPFFR1 knockout male mice demonstrated reduced spontaneous food intake and suppressed leptin- and ghrelin-induced feeding. In contrast, food intake was unaltered in NPFFR1 knockout female mice. Ablation of NPFFR1 did not cause any overt alterations in body weight of male mice but increased body weight in female mice associated with increased fat mass. NPFFR1 ablation worsened glucose tolerance and insulin sensitivity caused by a high fat diet in male but not in female mice.1NPFF has been widely recognized as an endogenous peptide that modulates the effects of opioids, with its ability to either block or potentiate morphine-induced analgesia depending on the site of administration. The increased presence of NPFF in the central nervous system (CNS) has been implicated in the development of opioid tolerance and dependence. Pharmacological blockade of NPFFR1/2 prevented the development of opioid-induced hyperalgesia and analgesic tolerance. Furthermore, the NPFF system has been shown to play a crucial role in modulating the effects of other drugs of abuse, as well as various physiological processes such as neuroendocrine and cardiovascular functions and energy homeostasis, including insulin release, food intake, memory, blood pressure, electrolyte balance, and neural regeneration. Consequently, the NPFF system has gained significant attention as a promising target for therapeutic interventions.1,3

Gene ID: 64107

UniProt: Q9EP86

Pathways: cAMP Metabolic Process