NPFFR2 antibody (Extracellular)

Catalog No. ABIN7581922

This Rabbit Polyclonal antibody specifically detects NPFFR2 in WB, FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for NPFFR2 antibody (Extracellular) (ABIN7581922)

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPFFR2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NPFFR2 Antibody

Binding Specificity: AA 186-199, Extracellular

Purpose: A Rabbit Polyclonal antibody to Neuropeptide FF Receptor 2 (extracellular)

Sequence: (C)EEKYYRVRLS SHNK

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - identical Human - 12 out of 14 amino acid residues identical

Characteristics: Anti-Neuropeptide FF Receptor 2 (extracellular) Antibody (ABIN7581922) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize NPFFR2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EEKYYRVRLSSHNK, corresponding to amino acid residues 186 - 199 of mouse NPFFR2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPFFR2

Target: NPFFR2 (NPFF2) (Neuropeptide FF Receptor 2 (NPFF2))

Alternative Name: NPFFR2

Background: NPFFR2, G-Protein Coupled Receptor 74, GPR74,Neuropeptide FF receptor 2, NPFFR1, and also known as GPR74, is a member of the G protein-coupled receptor superfamily containing two subtypes, NPFFR1 and NPFFR2.1-2NPFFR1 and NPFFR2 are the high affinity receptors the endogenous neuropeptides NPFF and NPAF, also known as RFamide peptides, which are characterized by their Arg-Phe-NH2 motif at the C-terminal end.1-2NPFFR2 is expressed in both the central and peripheral nervous system, as well as in other organs such as heart, kidney and adipose tissue macrophages.1-4NPFFR2 has been extensively studied for its role in pain modulation. Mice over-expressing NPFFR2 showed more sensitivity to mechanical and thermal noxious stimuli. In addition, a role in the modulation of the development of opioid tolerance and the regulation of withdrawal symptoms, has been suggested.3,5NPFFR2 signaling has been implicated in the regulation of energy homeostasis, through the inhibition of food intake and the modulation of metabolic rate and energy expenditure.2,3,6 In this regard, it is interesting to note that, NPFFR2 has been identified in adipose tissue macrophages, cells that are involved in the development of obesity-induced metabolic diseases.7

Gene ID: 104443

UniProt: Q924H0

Pathways: cAMP Metabolic Process