NPSR1 antibody (Extracellular)

Catalog No. ABIN7581923

The Rabbit Polyclonal anti-NPSR1 antibody has been validated for WB, IHC, FACS and LCI. It is suitable to detect NPSR1 in samples from Mouse.

Quick Overview for NPSR1 antibody (Extracellular) (ABIN7581923)

Target: NPSR1 (Neuropeptide S Receptor 1 (NPSR1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NPSR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NPSR1 Antibody

Binding Specificity: AA 297-312, Extracellular

Purpose: A Rabbit Polyclonal antibody to Neuropeptide S Receptor (extracellular)

Sequence: (C)DNFNVLPDTK ERFYAS

Specificity: Extracellular, 3rd loop.

Predicted Reactivity: Rat - 15 out of 16 amino acid residues identical,Human - 14 out of 16 amino acid residues identical

Characteristics: Anti-Neuropeptide S Receptor (extracellular) Antibody (ABIN7581923) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize NPSR1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DNFNVLPDTKERFYAS, corresponding to amino acid residues 297 - 312 of mouse NPSR1 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NPSR1

Target: NPSR1 (Neuropeptide S Receptor 1 (NPSR1))

Alternative Name: NPSR1

Background: NPSR1, G-Protein Coupled Receptor 154, GPR154, G-Protein Coupled Receptor for Asthma Susceptibility, GPRA,The Neuropeptide S Receptor (NPSR1), also known as GPR154, GPRA, or VRR1, belongs to the G protein-coupled receptor (GPCR) superfamily and is activated by neuropeptide S (NPS)1,2.NPS is a 20-residue peptide, named due to the presence of a serine (S) residue in the N-terminal position of the molecule. NPS expression is limited to a few discrete brain areas, such as in isolated cells of the amygdala, the hypothalamus, and specific regions of the brainstem. The primary sequence of NPS is highly conserved among vertebrates especially at the N-terminus, which is crucial for biological activity. The positions 2, 3, 4, and 5 on N-terminus of NPS play important roles in bioactivity and activation of NPSR11,2.The Neuropeptide S Receptor is widely distributed in the brain particularly in regions that are associated with regulation of stress response, memory, the olfactory system, and regulation of arousal. NPSR1 can signal via both Gq and Gs to increase cellular excitability and inhibit cell growth. Via the Gq pathway, NPSR1 leads to activation of Gq-phospholipase C and cleavage of phospholipid phosphatidylinositol 4,5-bisphosphate to inositol 1,4,5-trisphosphate (IP3) and diacylglycerol. IP3 activates IP3 receptor and releases Ca2 + from the ER. In some neurons, store-operated calcium channels are also activated, allowing entry of extracellular calcium into the cells 2.In the Gs pathway, NPSR signaling activates adenylate cyclase, increased synthesis of cAMP, and activation of protein kinase A 2.NPSR1 activation, like other GPCRs, also leads to activation of extracellular signal-regulated kinase 1/2 (ERK1/2) 2. Knockout studies in mice demonstrated that the NPS system produces anti-aggressive effects, reduces social anxiety behavior, modulates fear response and enhances motor performance skills3,4. A growing body of evidence suggests a close relationship between neuropeptide S and its receptor NPSR1 system in Parkinson's disease (PD). Indeed, administration of NPS to the central nervous syatem, ameliorates motor and non-motor dysfunctions in PD models, suggesting that targeting the NPS/NPSR1 system might be beneficial for Parkinson-related signs and symptoms 5.

Gene ID: 319239

UniProt: Q8BZP8