PACC1 (AA 95-110), (Extracellular) antibody

Catalog No. ABIN7581942

The Rabbit Polyclonal anti-PACC1 antibody (ABIN7581942) specifically detects PACC1 in FACS, IHC, LCI and WB. The antibody is reactive with Mouse samples.

Quick Overview for PACC1 (AA 95-110), (Extracellular) antibody (ABIN7581942)

Target: PACC1

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI), Western Blotting (WB)

Product Details

Binding Specificity: AA 95-110, Extracellular

Purpose: A Rabbit Polyclonal antibody to PACC1/TMEM206 (extracellular)

Sequence: (C)KLKHPVMSVS YKEVDR

Specificity: Extracellular loop.

Predicted Reactivity: Rat,human - identical

Characteristics: Anti-PACC1/TMEM206 (extracellular) Antibody (ABIN7581942) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize PACC1 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KLKHPVMSVSYKEVDR, corresponding to amino acid residues 95 - 110 of mouse PACC1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details

Target: PACC1

Background: Proton-Activated Chloride Channel, PAC, Transmembrane Protein 206, Proton Activated Chloride Channel 1, Acid-Sensitive Outwardly-Rectifying Anion Channel, ASOR,The proton-activated Cl- (PAC) channel, which opens by extracellular acidic pH , has been well characterized in several cells and tissues by electrophysiology recordings, although its molecular identity was unknown.Recently, two independent groups, identified a two-transmembrane protein named PACC1 (or TMEM206) as the molecular underlie of the proton-activated Cl- channel 1,2.PACC1 has no discernible sequence homology with previously characterized Cl- channels, although its structure of two transmembrane domains with cytoplasmic N- and C-termini and a large extracellular loop, resembles that of the Na+ -selective channels ASICs and ENaCs. In fact, recent work suggests that the functional unit of the proton-activated Cl- channel is a trimeric form of PACC1 subunits3,4.PAC activity that is stimulated by the lowering of extracellular pH and has been recorded in a wide range of mammalian cells and tissues. Acidic pH is crucial for the function of intracellular organelles in the secretory and endocytic pathways and is also one of the pathological hallmarks of many diseases, including cerebral and cardiac ischemia, cancer, infection and inflammation. Therefore, it is no surprise that the involvement of PACC1 in several physiological processes of the endocytic pathway5,6 and pathological conditions such as acid-induced cell death and brain damage after ischemic stroke7 or that upregulation of PACC1 is associated with poor prognosis in patients with hepatocellular carcinoma8.

Gene ID: 66950

UniProt: Q9D771