PKD2 antibody (Intracellular)

Catalog No. ABIN7581944

The Rabbit Polyclonal anti-PKD2 antibody has been validated for WB, IHC, IF and IC. It is suitable to detect PKD2 in samples from Human.

Quick Overview for PKD2 antibody (Intracellular) (ABIN7581944)

Target: PKD2 (Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKD2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Product Details anti-PKD2 Antibody

Binding Specificity: AA 914-927, Intracellular

Purpose: A Rabbit Polyclonal Antibody to TRPP1 (PKD2) Channel

Sequence: (C)ERWESDDAAS QISH

Specificity: Intracellular, C-terminus

Predicted Reactivity: 14 amino acid residues identical, rat - 11,Mouse - 12

Characteristics: Anti-TRPP1 (PKD2) Antibody (ABIN7581944) is a highly specific antibody directed against an epitope of human Polycystin-2. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize TRPP1 from human, rat, and mouse samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ERWESDDAASQISH, corresponding to amino acid residues 914-927 of human TRPP1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for PKD2

Target: PKD2 (Polycystic Kidney Disease 2 (Autosomal Dominant) (PKD2))

Alternative Name: PKD2

Background: Polycystin-2, Polycystic kidney disease 2 protein, PC2,Transient receptor potential (TRP) channels are relatively non-selective ion channels that enable the flow of cations down their electrochemical gradient. This enables the increase in intracellular Na+ and Ca2+ concentrations and ultimately in the cell membrane depolarization, which is important for action potential propagation and muscle contraction1. They are activated by a broad range of stimuli, namely temperature, voltage, pH , endocrine factors, as well as signaling molecules2.The TRP channel family is composed of 28 members divided into 7 subgroups: TRPV, TRPC, TRPM, TRPA, TRPN, TRPP and TRPML. All members of the TRP family have 6 transmembrane (TM) domains, with the pore between the fifth (S5) and sixth (S6) TM domains. In general, TRP channels enable the passage of either Na+ or Ca2+ ions with little to no preference.However, some channels do exhibit some selectivity. Also, TRP channels do not display the positive charges in the S4 voltage-sensing domain like most voltage-sensitive channels, although they do display voltage-dependency3. In addition, the C-terminal, located intracellularly, contains a TRP domain comprising 25 amino acids that are highly conserved between TRP channels. Within the TRP domain, there is a TRP box composed of six amino acids, and TRP box 2 - a proline rich domain1,3. The TRP domain seems to be responsible for the binding of PIP2, a phospholipid important for the regulation of channel activity4.TRPP1 (polycystin-2, PC2, PKD2) belongs to the TRPP subfamily along with TRPP3 and TRPP5 proteins, and forms non-selective cation channels with different permeability to various divalent cations5,6. The cellular localization of TRPP1 has been and still is the subject of a lasting debate. In many cell-types, TRPP1 is retained in the endoplasmic reticulum (ER) where it most likely functions as a Ca2+ release channel7,8, and with the help of cofactors, TRPP2 reaches the plasma membrane and the cilia8. TRPP1 expression is widespread and is best characterized for its expression in the kidney where it is developmentally regulated9,10. In the kidney, it associates with PKD1 (TRPP2) an eleven TM-spanning protein (which does not belong to the TRP superfamily) to form functional channels11. In addition, TRPP1 is identified as one of the genes responsible for autosomal dominant polycystic kidney disease (ADPKD)5,12.

Gene ID: 5311

UniProt: Q13563

Pathways: cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity