Periaxin antibody (Intracellular)

Catalog No. ABIN7581946

This Guinea Pig Polyclonal antibody specifically detects Periaxin in WB and IHC. It exhibits reactivity toward Rat.

Quick Overview for Periaxin antibody (Intracellular) (ABIN7581946)

Target: Periaxin (PRX)

Reactivity: Rat

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Periaxin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-Periaxin Antibody

Binding Specificity: AA 155-170, Intracellular

Purpose: A Guinea Pig Polyclonal Antibody to Periaxin

Sequence: (C)DVEFSFPKFS RLRR

Specificity: Intracellular, N-terminus

Predicted Reactivity: Mouse,human - identical

Characteristics: Guinea pig Anti-Periaxin Antibody (ABIN7581946), formerly ), raised in guinea pig, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Periaxin from mouse, rat and human samples. The antigen used to immunize guinea pigs is the same as Anti-Periaxin Antibody (ABIN7043432, ABIN7044712 and ABIN7044713) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVEFSFPKFSRLRR, corresponding to amino acid residues 155-170 of rat PRX

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:600

Comment:

Negative Control: (ABIN7236245)

Blocking Peptide: (ABIN7236245)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Periaxin

Target: Periaxin (PRX)

Alternative Name: PRX

Background: PRX, CMT4F,Periaxin protein is a membrane-associated protein expressed in myelinating Schwann cells. It is required for the stable maintenance and development of a normal myelin sheath in the peripheral nervous system. It is also a member of cytoskeletal complexes in lens fibers where it plays an important role in maturation, packing, and membrane organization of lens fiber cell1,2. The periaxin gene (PRX) encodes two protein isoforms, L-periaxin and S-periaxin generated by alternative mRNA splicing. These proteins contain N-terminal PDZ domains (PSD-95/Discs-large/ZO-1) important for organizing protein-protein interactions.L-periaxin is a component of the dystroglycan-dystrophin-related protein-2 complex (DRP2) linking the Schwann cell cytoskeleton to the extracellular matrix. Its location changes during myelination, at first the protein is localized to the adaxonal plasma membrane and later, in the abaxonal plasma membrane. S-periaxin is distributed in a uniform manner in the cytoplasm and the nucleus of the Schwann cell1-3. Mutations in the Periaxin gene are known to cause autosomal recessive demyelinating in Type 4F Charcot-Marie-Tooth (CMT4F) and Dejerine-Sottas disease3.Periaxin knockout mice develop normally myelinated peripheral nervous system at first but develop a late onset demyelinating peripheral neuropathy1.

Gene ID: 78960

UniProt: Q63425