SCN7A antibody (Intracellular)

Catalog No. ABIN7581963

The Rabbit Polyclonal anti-SCN7A antibody (ABIN7581963) specifically detects SCN7A in WB and IHC. The antibody is reactive with Mouse samples.

Quick Overview for SCN7A antibody (Intracellular) (ABIN7581963)

Target: SCN7A (Sodium Channel, Voltage-Gated, Type VII, alpha Subunit (SCN7A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SCN7A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SCN7A Antibody

Binding Specificity: AA 842-856, Intracellular

Purpose: A Rabbit Polyclonal antibody to SCN7A (NaX)

Sequence: (C)ESDIENLDNK ETRSK

Specificity: Intracellular, 6th loop.

Predicted Reactivity: Rat- identical,Human - 13 out of 15 amino acid residues identical

Characteristics: Anti-SCN7A (NaX) Antibody (ABIN7581963) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SCN7A from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)ESDIENLDNKETRSK, corresponding to amino acid residues 842 - 856 of mouse SCN7A 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SCN7A

Target: SCN7A (Sodium Channel, Voltage-Gated, Type VII, alpha Subunit (SCN7A))

Alternative Name: SCN7A

Background: Sodium Channel Protein Type 7 Subunit Alpha, Putative voltage-gated sodium channel subunit alpha Nax, SCN6A, Nav2.1, Nav2.2,SCN7A, also known as NaX, Nav2.1 or Nav2.3, is an atypical member of the SCNA family of voltage-dependent sodium channels, that includes nine mammalian members: Nav1.1 through Nav1.9.As its name implies, SCN7A was initially identified as a new member of the Nav channel family. However, atypical sequence features, namely fewer positive charged residues in the S4 voltage sensor domain than other sodium channels, and the inability to record voltage-activated sodium currents, raised speculation that this channel might have distinct physiological roles. 1,2Indeed, a role of SCN7A as a sensor of extracellular sodium concentrations, has been described.SCN7A is expressed both in the central nervous system and in peripheral organs. In the brain, SCN7A expression appears in both neurons and glia cells. Experiments with SCN7A knockout mice, showed that SCN7A might function as an extracellular sodium sensor. Knockout mice demonstrated abnormal saline-intake behaviour under conditions of water deprivation, as well as abnormal blood pressure changes after salt intake. 2,3,4In addition, expression of SCN7A in epithelial tissues has been linked to epithelial homeostasis following injury. Water loss following skin disorders or injury, will result in an increase in extracellular sodium concentration and hence, will be able to stimulate SCN7A function. Indeed, blocking of SCN7A function in epithelial tissues in animal models, showed a decrease in scarring and atopic dermatitis-like symptoms. 2,5,6

Gene ID: 20272

UniProt: Q62467