MCT3 antibody (Intracellular)

Catalog No. ABIN7581969

This anti-MCT3 antibody is a Rabbit Polyclonal antibody detecting MCT3 in WB. Suitable for Rat.

Quick Overview for MCT3 antibody (Intracellular) (ABIN7581969)

Target: MCT3 (Monocarboxylate Transporter 3 (MCT3))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCT3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MCT3 Antibody

Binding Specificity: AA 421-434, Intracellular

Purpose: A Rabbit Polyclonal Antibody to Monocarboxylate Transporter 3

Sequence: (C)RSAEGGASDP EDVE

Specificity: Intracellular, C-terminus

Cross-Reactivity (Details): Not recommended for human samples.

Predicted Reactivity: Mouse - identical

Characteristics: Anti-MCT3 Antibody (ABIN7581969) is a highly specific antibody directed against an epitope of the rat Monocarboxylate transporter 3. The antibody can be used in western blot analysis. It has been designed to recognize MCT3 from rat and mouse samples. The antibody is unlikely to recognize the protein from human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RSAEGGASDPEDVE, corresponding to amino acid residues 421-434 of rat MCT3

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MCT3

Target: MCT3 (Monocarboxylate Transporter 3 (MCT3))

Alternative Name: SLC16A8

Background: Monocarboxylate transporter 3, Monocarboxylic acid transporter 3, SLC16A8,Proton-linked monocarboxylate transporters (MCTs) are a family of highly homologous trans-membrane symporters, involved in the transportation of monocarboxylic acids across the plasma membrane such as: lactate, pyruvate, branched-chain oxoacids derived from leucine, valine, and isoleucine, ketone bodies, acetoacetate, β-hydroxybutyrate and acetate. Thus, MCTs play an important role in metabolic communication among cells and in mammalian metabolism.MCT3 is encoded by the Slc16a8 gene and contains at least nine members in humans1. Structurally, MCTs have 12 transmembrane spanning domains with cytoplasmic NH2- and COOH-terminals. The COOH-terminal regions are not well conserved among the MCTs isoforms while the membrane spanning domains share the greatest sequence identity3,4. Findings suggest that the N-terminal domain is more important for energy coupling, membrane insertion and correct structure maintenance, whereas the C-terminal domain is more important for the determination of substrate specificity1.MCT3 is expressed in the retinal pigment epithelium, preferentially expressed in the basolateral membrane in the adult and in choroid plexus epithelium where it plays a role in regulating pH and lactate concentrations in the outer retina. Reduced levels of MCT3 in response to trauma or disease could contribute to pathologic changes in the retina2,3. MCT3 can form a heteromeric complex with the accessory protein CD147, an immunoglobulin superfamily protein.

Gene ID: 65200

UniProt: O70461