SLC25A22 antibody (Intracellular)

Catalog No. ABIN7581974

The Rabbit Polyclonal anti-SLC25A22 antibody is suitable to detect SLC25A22 in samples from Human. It has been validated for WB and IHC.

Quick Overview for SLC25A22 antibody (Intracellular) (ABIN7581974)

Target: SLC25A22 (Solute Carrier Family 25 (Mitochondrial Carrier: Glutamate), Member 22 (SLC25A22))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC25A22 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLC25A22 Antibody

Binding Specificity: AA 91-106, Intracellular

Purpose: A Rabbit Polyclonal antibody to SLC25A22 (GC1)

Sequence: (C)RHQLSKDGQK LTLLKE

Specificity: Intracellular, 1st loop

Predicted Reactivity: Rat,mouse - 15 out of 16 amino acid residues identical

Characteristics: Anti-SLC25A22 (GC1) Antibody (ABIN7581974) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLC25A22 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RHQLSKDGQKLTLLKE, corresponding to amino acid residues 91 - 106 of human SLC25A22

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

Application Dilutions Western blot wb: 1:200-1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC25A22

Target: SLC25A22 (Solute Carrier Family 25 (Mitochondrial Carrier: Glutamate), Member 22 (SLC25A22))

Alternative Name: SLC25A22

Background: Mitochondrial glutamate carrier 1, Glutamate/H(+) Symporter 1, Solute carrier family 25 member 22,SLC25A22- solute carrier family 25 member 22, also known as Mitochondrial glutamate carrier 1 (GC1), is a mitochondrial glutamate/H+ symporter, responsible for the transport of glutamate from the cytosol into the mitochondrial matrix with the concomitant import of a proton1. SLC25A22 mutations has been associate with colorectal cancer, osteosarcoma and neonatal myoclonic epilepsy2. SLC25A22 was shown to be expressed in different tissues, especially in the brain, liver, and pancreas.The mitochondrial carriers are a family of transport proteins in the inner membranes of mitochondria. They shuttle substrates, metabolites, and cofactors through this membrane and connect cytoplasm functions with others in the matrix. SLC25 is a large family of nuclear-encoded transporters embedded in the inner mitochondrial membrane and in a few cases other organelle membranes. The two isoforms of the glutamate carrier GC1 and GC2 (encoded by SLC25A22 and SLC25A18, respectively) catalyze the transport of glutamate across the inner mitochondrial membrane, either by proton (H+) co-transport or in exchange for hydroxyl ions3 (OH-).SLC25A22 has a tumor-promoting function, promoting proliferation and migration of colorectal cancer cells with mutant KRAS, and formation and metastasis of colorectal cancer xenograft tumors in mice. Patients with colorectal tumors that express increased levels of SLC25A22 have shorter survival times than patients whose tumors have lower levels. SLC25A22 induces intracellular synthesis of aspartate, activation of mitogen-activated protein kinase and extracellular signal-regulated kinase signaling and reduces oxidative stress4.SLC25A22 has been identified as the underlying cause of early myoclonic encephalopathy (EME) an electro-clinical epilepsy syndrome that manifests itself within the neonatal period or within the first 3 months of life. Genetic mapping of an autosomal recessive form of this condition to chromosome 11p15.5 and the identification of a missense mutation (p.Pro206Leu) in the gene encoding SLC25A22. The mutation cosegregated with the disease and altered a highly conserved amino acid, resulting in severe neonatal epilepsies with suppression-burst pattern3.

Gene ID: 79751

UniProt: Q9H936

Pathways: Dicarboxylic Acid Transport