SLIT1 antibody (Secreted)

Catalog No. ABIN7581986

The Rabbit Polyclonal anti-SLIT1 antibody has been validated for WB and IHC. It is suitable to detect SLIT1 in samples from Mouse.

Quick Overview for SLIT1 antibody (Secreted) (ABIN7581986)

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLIT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SLIT1 Antibody

Binding Specificity: AA 1170-1184, Secreted

Purpose: A Rabbit Polyclonal antibody to SLIT1

Sequence: (C)RDTYLQFTDL QNWPR

Specificity: Secreted

Cross-Reactivity (Details): This antibody is specific to SLIT1, it won't recognize the highly homologous protein SLIT2.

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-SLIT1 Antibody (ABIN7581986) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize SLIT1 from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RDTYLQFTDLQNWPR, corresponding to amino acid residues 1170 - 1184 of mouse SLIT1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLIT1

Target: SLIT1 (Slit Homolog 1 (SLIT1))

Alternative Name: SLIT1

Background: Slit Guidance Ligand 1, Slit Homolog 1 Protein, Multiple EGF-Like Domains Protein 4, MEGF4,Slit homolog 1 protein (SLIT1), also known as multiple EGF-like domains protein 4, and MEGF4, is a secreted protein member of the SLIT family involved in axon guidance and cell migration.SLIT1 is a member of the SLIT family proteins that includes three members: SLIT1, SLIT2 and SLIT3. SLITS are secreted glycoproteins comprised of four stretches of leucine-rich repeat (LRR) domains, nine epidermal growth factor (EGF) repeats, a Laminin-G-like domain, and a C-terminal cysteine knot.1,2SLITS exert their many physiological roles by binding to Roundabout (Robo) receptors family, that includes four members (Robo 1 to Robo 4), which are single transmembrane receptors belonging to the immunoglobulin superfamily.1,2SLITS play a crucial role in axon guidance during the development of the nervous system. Axon guidance is the process by which neurons extend their axons to reach their appropriate targets, such as other neurons, muscles, or glands. This process is essential for the formation of functional neural circuits.1,3In addition, SLIT1 is involved in various aspects of the regulation of neural progenitor cells (NPCs). NPCs are the precursors to neurons and glial cells in the nervous system. They undergo proliferation, migration, and differentiation to generate the diverse cell types found in the mature brain. SLIT1 is involved in several aspects of NPCs functions, including promoting the proliferation of NPCs in several brain regions, guiding NPCs from the sub-ventricular zone towards the cortical plate and supporting the differentiation of NPCs into specific neuronal or glial cell types.3,4Finally, a complex role for SLIT1 in cancer has emerged. SLIT1 has been ascribed a tumor suppressive role in certain cancers such as breast and colorectal, while in others it appears to associate with tumor progression. In particular, in glioma and pancreatic cancer, SLIT1 expression has been correlated with enhanced metastasis formation and poor prognosis. 2,5,6

Gene ID: 20562

UniProt: Q80TR4

Pathways: Regulation of Cell Size