SV2A antibody (Intracellular)

Catalog No. ABIN7581988

This Rabbit Polyclonal antibody specifically detects SV2A in WB, IHC and IP. It exhibits reactivity toward Mouse.

Quick Overview for SV2A antibody (Intracellular) (ABIN7581988)

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SV2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-SV2A Antibody

Binding Specificity: AA 109-123, Intracellular

Purpose: A Rabbit Polyclonal Antibody to SV2A

Sequence: (C)KGERMADGAP LAGVR

Specificity: Intracellular, N-terminus.

Predicted Reactivity: Rat,human- identical

Characteristics: Anti-SV2A Antibody (ABIN7581988) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize SV2A from rat, mouse and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KGERMADGAPLAGVR, corresponding to amino acid residues 109 - 123 of mouse SV2A

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SV2A

Target: SV2A (Synaptic Vesicle Glycoprotein 2A (SV2A))

Alternative Name: SV2A

Background: Synaptic vesicle glycoprotein 2A, Synaptic vesicle protein 2A,SV2A, also known as Synaptic vesicle glycoprotein 2A and Synaptic vesicle protein 2A, is an integral membrane glycoprotein located on synaptic vesicles, which are responsible for storing and releasing neurotransmitters in the brain.1,2SV2A is a member of a protein family that also includes SV2B and SV2C. All three proteins exhibit a similar topology of 12 transmembrane domains, but have different expression patterns in the brain. SV2A is ubiquitously expressed and is found in virtually all types of neurons throughout the brain, while SV2B has a more restricted expression pattern, being present in certain areas of the brain such as the hippocampus and cortex. SV2C displays a more limited expression, and is primarily found in the basal ganglia and some brainstem nuclei.1,2The exact physiological role of SV2A is not clear, but a role in the regulation of neurotransmitter release, maintenance of synaptic vesicle pool and modulation of synaptic plasticity, have been suggested. 1,3Interestingly, the role of SV2A in pathological conditions, has received considerable attention. Animals homozygous for the SV2A gene appear normal at birth, however, they fail to grow, experience severe seizures, and die within 3 weeks. 4In fact, SV2A was identified as the binding site for the antiepileptic drug levetiracetam, which is effective in reducing seizure frequency in epilepsy patients.1,3In addition, changes in SV2A expression have been observed in neurodegenerative diseases like Alzheimer's, schizophrenia, Parkinson's disease and dementia. 1,3Changes in SV2A expression are increasingly recognized as a valuable biomarker for synaptic density, a parameter providing insights into the integrity and function of synapses in the brain. Synaptic density refers to the number of synapses in a given volume of brain tissue and is a critical indicator of neuronal health and connectivity. For this purpose, radioligands that specifically bind to SV2A and can be used in Positron Emission Tomography (PET) scans, have been developed to quantify synaptic density non-invasively. Thus, measuring SV2A expression is a promising tool for diagnosing and monitoring neurological and psychiatric conditions, ultimately aiding in the development of more effective treatments.1,5,6

Gene ID: 64051

UniProt: Q9JIS5