Tetraspanin 2 antibody (Extracellular)

Catalog No. ABIN7581998

The Rabbit Polyclonal anti-Tetraspanin 2 antibody has been validated for WB, FACS, IHC and LCI. It is suitable to detect Tetraspanin 2 in samples from Mouse.

Quick Overview for Tetraspanin 2 antibody (Extracellular) (ABIN7581998)

Target: Tetraspanin 2 (TSPAN2)

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Tetraspanin 2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Live Cell Imaging (LCI)

Product Details anti-Tetraspanin 2 Antibody

Binding Specificity: AA 114-130, Extracellular

Purpose: A Rabbit Polyclonal antibody to Tetraspanin 2 (extracellular)

Sequence: (C)DVAIRHVQSM YEEAYSD

Specificity: Extracellular, 2nd loop.

Predicted Reactivity: Rat - identical,Human - 14 out of 17 amino acid residues identical

Characteristics: Anti-Tetraspanin 2 (extracellular) Antibody (ABIN7581998) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize TSPAN2 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)DVAIRHVQSMYEEAYSD, corresponding to amino acid residues 114 - 130 of mouse TSPAN2

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.2 mL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for Tetraspanin 2

Target: Tetraspanin 2 (TSPAN2)

Alternative Name: TSPAN2

Background: Tetraspan NET-3, TSPAN2,Tetraspanin 2 (TSPAN2) is a member of the Tetraspanin family of proteins, that comprises 33 members in humans. The family is characterized by the presence of four transmembrane domains, which give rise to the name "tetraspanin", as well as the presence two extracellular loops (EC1 and EC2), a small cytoplasmic loop, and short N- and C-terminal tails 1,2.Tetraspanins are able to interact with each other and also with numerous partner proteins such as integrins, growth factor receptors and ion channels, to create a dynamic network of interactions known as the tetraspanin-enriched microdomains (TEMs). Consequently, tetraspanins are regarded as molecular organizers and by forming complexes with these proteins, tetraspanins can modulate signal transduction pathways and cellular responses 2,3,4.Although TSPAN2 shares a relatively high homology with well-studied tetraspanins like CD9 (TSPAN29) or CD81 (TSPAN28), it has been less well-characterised up until now 3.Nevertheless, TSPAN2, like the related CD9 and CD81 proteins, has been described in relation to exosome formation, specifically regarding neuroinflammation and microglia activation 5, as well as oligodendrocyte differentiation into myelin-forming glia and in the progression of tumor metastasis by modulating cancer cell motility and invasion 3,5.

Gene ID: 70747

UniProt: Q922J6