ADORA1 antibody (Extracellular, Loop 2) (FITC)

Catalog No. ABIN7582002

The FITC-conjugated Rabbit Polyclonal anti-ADORA1 antibody (ABIN7582002) specifically detects ADORA1 in FACS and LCI. The antibody is reactive with Rat samples.

Quick Overview for ADORA1 antibody (Extracellular, Loop 2) (FITC) (ABIN7582002)

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADORA1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-ADORA1 Antibody (FITC)

Binding Specificity: AA 153-168, Extracellular, Loop 2

Purpose: A Rabbit Polyclonal Antibody to Adenosine A1 Receptor (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)EQDWRANGSV GEPVIK

Predicted Reactivity: Mouse - 14 out of 16 amino acid residues identical Not recommended for human samples

Characteristics: Anti-Adenosine A1 Receptor (extracellular) Antibody (ABIN7581822) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize Adenosine A1 Receptor from mouse and rat samples. The antibody is not recommended for human samples. Anti-Adenosine A1 Receptor (extracellular)-FITC Antibody (ABIN7581822)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)EQDWRANGSVGEPVIK, corresponding to amino acid residues 153 - 168 of rat Adenosine A1 Receptor 

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for ADORA1

Target: ADORA1 (Adenosine A1 Receptor (ADORA1))

Alternative Name: ADORA1

Background:

Synonyms: Adenosine Receptor A1, Adenosine A1R, ADORA1

Description: Adenosine is an endogenous nucleoside generated locally in tissues under conditions of hypoxia, ischemia, or inflammation. It modulates a variety of physiological functions in many tissues including the brain and heart.1,2 Adenosine exerts its actions via four specific adenosine receptors (also named P1 purinergic receptors): Adenosine A1 Receptor (A1AR), Adenosine A2A Receptor (A2AAR), Adenosine A2B Receptor (A2BAR), and Adenosine A3 Receptor (A3AR). All are integral membrane proteins and are members of the G protein-coupled receptor superfamily. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, a cytoplasmic carboxyl terminus, and a third intracellular loop that is important for binding G proteins.1-3 The adenosine receptors can be distinguished on the basis of their differential selectivity for adenosine analogs.1-3A1AR is widely distributed and has been well characterized. High expression of A1AR is found in the brain (mainly in the cortex, cerebellum, and hippocampus), dorsal horn of the spinal cord, adrenal gland, and atria, and to a lower extent in several other tissues including adipose tissue, the colon, and kidney.2,4A1AR modulates the activity of several ion channels. Activation of A1AR (by adenosine, its major agonist) inhibits N-type Ca2+ channels via a voltage-dependent, pertussis toxin (PTX)-sensitive pathway in neurons of the rat major pelvic ganglia (MPG).5Since A1AR is the most prominent adenosine receptor in adipocytes, it has become a natural target for research on obesity, which is a major health problem.6,7 A possible role in cell proliferation and carcinogenesis has also been suggested for A1AR.8,9

Gene ID: 29290

UniProt: P25099

Pathways: EGFR Signaling Pathway, Negative Regulation of Hormone Secretion, Synaptic Membrane