CALHM2 antibody (Extracellular, Loop 2) (FITC)

Catalog No. ABIN7582013

The Rabbit Polyclonal anti-CALHM2 antibody is suitable to detect CALHM2 in samples from Mouse. It has been validated for FACS and LCI.

Quick Overview for CALHM2 antibody (Extracellular, Loop 2) (FITC) (ABIN7582013)

Target: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CALHM2 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-CALHM2 Antibody (FITC)

Binding Specificity: AA 133-146, Extracellular, Loop 2

Purpose: A Rabbit Polyclonal antibody to CALHM2 (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)SEFVDPSSLT AGDK

Predicted Reactivity: Rat - 13 out of 14 amino acid residues identical Human - 11 out of 14 amino acid residues identical

Characteristics: Anti-CALHM2 (extracellular) Antibody (ABIN7581840) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize CALHM2 from mouse, rat and human samples. Anti-CALHM2 (extracellular)-FITC Antibody (ABIN7581840)-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SEFVDPSSLTAGDK, corresponding to amino acid residues 133 - 146 of mouse CALHM2

Isotype: IgG

Application Details

Application Notes:

WB: The optimal concentration should be determined by the user

FC: 2.5-5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for CALHM2

Target: CALHM2 (Calcium Homeostasis Modulator 2 (CALHM2))

Alternative Name: CALHM2

Background:

Synonyms: Calcium homeostasis modulator protein 2, Protein FAM26B, FAM26B

Description: Calcium homeostasis modulator protein 2 (CALHM2) is a member of the CALHM channel family, a group of transmembrane proteins that play essential roles in calcium ion transport and homeostasis. The CALHM family consist in humans of six members: CALHM1 to CALHM6.1CALHM2 functions as a calcium-permeable ion channel. It allows calcium ions to pass through the cell membrane in response to specific stimuli, such as changes in membrane potential or extracellular calcium concentration.2,3CALHM2 is composed of 323 amino acids and assembles as an undecamer with each protomer containing a large N-terminal transmembrane (TM) domain with 4 TM helices, an intracellular C-terminal domain, and a small extracellular linker region. 2,3CALHMs permeate ions and ATP in a voltage-dependent manner to modulate neuronal excitability. CALHM2 has been shown to accommodate lipids in its hydrophobic channel in order to regulate channel function.2,3Ion and ATP permeation by CALHM proteins play a role in the physiology of cognition, depression, gustatory signaling, and the pathology of Alzheimer's disease.Specifically, CALHM2 expression was increased in Alzheimer's disease (AD) patients and in an AD model in mice. Knockout of CALHM2 improved Aβ plaque deposition, reduced neuroinflammation, and improved cognitive impairment in an AD mice model.4Moreover, microglial CALHM2 may have a central role in both chronic inflammatory diseases (like AD) and in acute inflammatory reactions and neuroinflammation.4

Gene ID: 72691

UniProt: Q8VEC4