GPR171 antibody (Extracellular) (PE)

Catalog No. ABIN7582033

This Rabbit Polyclonal antibody specifically detects GPR171 in FACS and LCI. It exhibits reactivity toward Mouse.

Quick Overview for GPR171 antibody (Extracellular) (PE) (ABIN7582033)

Target: GPR171 (G Protein-Coupled Receptor 171 (GPR171))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR171 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR171 Antibody (PE)

Binding Specificity: AA 155-169, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR171 (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)

Sequence: Peptide (C)KDIKEKSNVG (S)MEFK

Specificity: 2nd extracellular loop

Predicted Reactivity: Mouse,15 amino acid residues identical, rat - 13,human - 14

Characteristics: Anti-GPR171 (extracellular) Antibody (ABIN7043150, ABIN7044447 and ABIN7044448) is a highly specific antibody directed against an extracellular epitope of the mouse protein. The antibody can be used in western blot, indirect flow cytometry, and live cell imaging applications. It has been designed to recognize GPR171 from human, mouse and rat samples. Anti-GPR171 (extracellular)-PE Antibody (ABIN7043150, ABIN7044447 and ABIN7044448-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171.

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7235380)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR171

Target: GPR171 (G Protein-Coupled Receptor 171 (GPR171))

Alternative Name: GPR171

Background: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963,G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN, one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.BigLEN-GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Gene ID: 229323

UniProt: Q8BG55