GPR183 antibody (Extracellular) (FITC)

Catalog No. ABIN7582034

The Rabbit Polyclonal anti-GPR183 antibody has been validated for FACS and LCI. It is suitable to detect GPR183 in samples from Human.

Quick Overview for GPR183 antibody (Extracellular) (FITC) (ABIN7582034)

Target: GPR183 (G Protein-Coupled Receptor 183 (GPR183))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR183 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-GPR183 Antibody (FITC)

Binding Specificity: AA 18-31, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR183 (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)GND(S)DLYAHHSTAR

Specificity: Extracellular, N-terminus.

Predicted Reactivity: Human - 13 out of 14 amino acid residues identical Mouse,rat - 12 out of 14 amino acid residues identical

Characteristics: Anti-GPR183 (extracellular) Antibody (ABIN7043152, ABIN7044457 and ABIN7044458) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize GPR183 from mouse, rat and human samples. Anti-GPR183 (extracellular)-FITC Antibody (ABIN7043152, ABIN7044457 and ABIN7044458-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize GPR183 from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GND(S)DLYAHHSTAR, corresponding to amino acid residues 18 - 31 of human GPR183 

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235392)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for GPR183

Target: GPR183 (G Protein-Coupled Receptor 183 (GPR183))

Alternative Name: GPR183

Background: G-Protein Coupled Receptor 183, Epstein-Barr Virus-Induced G-Protein Coupled Receptor 2, EBI2,G-protein coupled receptor 183 (GPR183) also known as Epstein-Barr virus-induced gene 2 (EBI2), is a chemotactic receptor that participates in the migratory capability of cells 2. It belongs to the rhodopsin-like subfamily of class A transmembrane spanning (7TM) G protein-coupled receptors and its most potent ligand is oxysterol 7α-25-OHC 1.Dihydroxycholesterols, such as oxysterol 7α-25-OHC, are generated from cholesterol in steady state but also in the context of inflammation. GPR183 senses these oxysterols and induces chemotactic migration of immune cells towards higher concentrations of these ligands and hence cells that express GPR183 are trafficking through an oxysterol gradient dependent manner acting like chemokine processes. The migratory function of this receptor affects several important immune processes, in particular, GPR183 is involved in the T-dependent antibody response in the germinal centers. This puts GPR183 in a unique position to effect autoimmunity and inflammatory bowel diseases 3,4.In addition, GPR183 is highly expressed by type 3 innate lymphoid cells (ILC3) and its oxysterol ligand was shown to be essential for the localization and the migration of ILC3s and to have a critical role for the formation of lymphoid tissues in the mouse colon during colitis 2.

Gene ID: 1880

UniProt: P32249