HRH1 antibody (Extracellular) (FITC)

Catalog No. ABIN7582039

This Rabbit Polyclonal antibody specifically detects HRH1 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for HRH1 antibody (Extracellular) (FITC) (ABIN7582039)

Target: HRH1 (Histamine Receptor H1 (HRH1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HRH1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-HRH1 Antibody (FITC)

Binding Specificity: AA 176-191, Extracellular

Purpose: A Rabbit Polyclonal Antibody to H1R (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)REDK(S)ETDFYNVTWF K

Specificity: 2nd extracellular loop.

Predicted Reactivity: Mouse, human - 14,16 amino acid residues identical,rat - 15

Characteristics: Anti-Histamine H1 Receptor/HRH1 (extracellular) Antibody (ABIN7043269, ABIN7044512 and ABIN7044513) is a highly specific antibody directed against an extracellular epitope of the mouse H1R. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize H1R  from mouse, rat and human samples. Anti-Histamine H1 Receptor/HRH1 (extracellular)-FITC Antibody (ABIN7043269, ABIN7044512 and ABIN7044513-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize H1R from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)REDK(S)ETDFYNVTWFK, corresponding to amino acid residues 176 - 191 of mouse H1 Histamine Receptor

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235747)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for HRH1

Target: HRH1 (Histamine Receptor H1 (HRH1))

Alternative Name: HRH1

Background: Histamine Receptor H1, H1R,Histamine, acts via distinct histamine H1, H2, H3, and H4 receptors, and regulates various physiological and pathological processes. Hence the activity of Histamine receptors is very crucial. They are members of G-protein coupled receptors superfamily. Thus, have seven transmembrane domains, extracellular N-terminal tail and a cytoplasmic C-terminus1.H1 receptor is excitatory and couples to Gq-type proteins, activating a signaling cascade which eventually leads to an increased cellular concentration of Ca2+ 3.H1 receptor is implicated in type I hypersensitivity allergic reactions caused by numerous allergens1. An allergic reaction occurs when allergens activate mast cells that release histamine, the released histamine binds to H1R and this causes an activation of a cascade that leads to vasodilation and vascular hyper-permeability, resulting in allergic inflammation.H1R is expressed in various tissues throughout body, including the airway, vascular smooth muscle, and brain2. H1R expressed in the brain plays an important role in the regulation of sleep-arousal cycle and memory by binding histamine where it acts as a neurotransmitter1.

Gene ID: 15465

UniProt: P70174

Pathways: Regulation of Carbohydrate Metabolic Process