KISS1R antibody (Extracellular) (FITC)

Catalog No. ABIN7582049

The Rabbit Polyclonal anti-KISS1R antibody has been validated for FACS and LCI. It is suitable to detect KISS1R in samples from Human.

Quick Overview for KISS1R antibody (Extracellular) (FITC) (ABIN7582049)

Target: KISS1R (KISS1 Receptor (KISS1R))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KISS1R antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-KISS1R Antibody (FITC)

Binding Specificity: AA 292-305, Extracellular

Purpose: A Rabbit Polyclonal Antibody to KISS1 Receptor (GPR54) (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)GSWHPRSYAA YALK

Specificity: 3rd extracellular loop

Predicted Reactivity: Rat - 13 out of 14 amino acid residues identical,Mouse - 12 out of 14 amino acid residues identical

Characteristics: Anti-KISS1 Receptor (GPR54) (extracellular) Antibody (ABIN7043294, ABIN7044548 and ABIN7044549) is a highly specific antibody directed against an epitope of human KISS1 receptor. The antibody can be used in western blot, immunohistochemistry and flow cytometry applications. It has been designed to recognize KISS1R from human, rat, and mouse samples. Anti-KISS1 Receptor (GPR54) (extracellular)-FITC Antibody (ABIN7043294, ABIN7044548 and ABIN7044549-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)GSWHPRSYAAYALK, corresponding to amino acid residues 292-305 of human KISS1R

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7235833)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for KISS1R

Target: KISS1R (KISS1 Receptor (KISS1R))

Alternative Name: KISS1R

Background: Kisspeptin receptor, KISS1R, Metastin receptor, AXOR12, G-Protein Coupled Receptor 54,The kisspeptin receptor (KISS1R, GPCR54) is a member of the G-protein coupled receptor superfamily. KISS1R natural ligands are peptides called kisspeptins which are encoded by the Kiss1 gene. The Kiss1 gene was originally discovered by Hershey scientists who named it after their famous chocolate, the "Kiss".The kisspeptins, acting through KISS1R, were found to be potent inhibitors of metastasis in a variety of cancer cell types including thyroid and pancreatic cancer cells. KISS1R and kisspeptins are involved in the direct regulation of the reproductive neuroendocrine axis.KISS1R is widely distributed throughout the brain, where it is most highly expressed in the hypothalamic and amygdaloid nuclei. High expression was also found in the pancreas and placenta. Lower-level expression was found in a variety of other peripheral tissues including peripheral blood leukocytes, kidney, lung, testes, spleen, thymus, adrenal glands and lymph nodes.1-3

Gene ID: 84634

UniProt: Q969F8