NrCAM antibody (Extracellular) (FITC)

Catalog No. ABIN7582053

This anti-NrCAM antibody is a Rabbit Polyclonal antibody detecting NrCAM in FACS and LCI. Suitable for Rat.

Quick Overview for NrCAM antibody (Extracellular) (FITC) (ABIN7582053)

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NrCAM antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-NrCAM Antibody (FITC)

Binding Specificity: AA 583-597, Extracellular

Purpose: A Rabbit Polyclonal Antibody to NrCAM (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)KDNGELPN DERFSVD

Specificity: Extracellular, N-terminus

Predicted Reactivity: human - 13,16 amino acid residues identical,Mouse - 15

Characteristics: Anti-NrCAM (extracellular) Antibody (ABIN7043369, ABIN7044690 and ABIN7044691) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NrCAM from mouse, rat and human samples. Anti-NrCAM (extracellular)-FITC Antibody (ABIN7043369, ABIN7044690 and ABIN7044691-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NrCAM from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KDNGELPN DERFSVD, corresponding to amino acid residues 583-597 of rat NrCAM

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7236074)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for NrCAM

Target: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Alternative Name: NRCAM

Background: Neuronal cell adhesion molecule, Nr-CAM, NgCAM-related cell adhesion molecule, Ankyrin-binding cell adhesion molecule, Neuronal surface protein Bravo, HBravo,NrCAM (Neuron-glia related cell adhesion molecule) is a cell adhesion molecule that belongs to the immunoglobulin-class (Ig) of the L1 family. NrCAM promotes axon growth and repulsion through trans homophilic binding of extracellular domains and intracellular coupling of the conserved cytoplasmic domains to the actin cytoskeleton. This interaction occurs through ankyrin B and ezrin-radixin-moesin adaptors. In addition, NrCAM is responsible for controlling dendritic spine densities, axonal guidance and targeting, and neurite outgrowth by acting as a co-receptor molecule at the neuronal cell surface1,2.NrCAM is highly expressed in the cortex1,3. Its structure contains a PDZ binding motif with the potential to bind PSD scaffold proteins PSD-95 and SAP102. The Ig domains of the protein mediate homophilic and heterophilic binding to multiple ligands2,3.Genetic polymorphisms in the NrCAM gene are linked to autism spectrum disorders, intellectual disability and addictive behavior. NrCAM knockout mice demonstrate autism spectrum disorder related behaviors including impaired sociability, cognitive inflexibility, hypersensitivity to sensory stimuli and altered visual cortical responses.

Gene ID: 497815

UniProt: P97686

Pathways: Regulation of Cell Size