CD73 antibody (Extracellular) (FITC)

Catalog No. ABIN7582054

This Rabbit Polyclonal antibody specifically detects CD73 in FACS. It exhibits reactivity toward Mouse.

Quick Overview for CD73 antibody (Extracellular) (FITC) (ABIN7582054)

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD73 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Product Details anti-CD73 Antibody (FITC)

Binding Specificity: AA 429-433, Extracellular

Purpose: A Rabbit Polyclonal Antibody to CD73 (extracellular) conjugated to the fluorescent dye FITC

Sequence: (C)KGSTLKKAFE HSVHR

Specificity: Extracellular, N-terminus

Predicted Reactivity: Human,rat - identical

Characteristics: Anti-CD73 (extracellular) Antibody (ABIN7042877, ABIN7044811 and ABIN7044812) is a highly specific antibody directed against an extracellular epitope of the mouse NT5E. The antibody can be used in western blot, immunohistochemistry and live cell flow cytometry applications. It has been designed to recognize NT5E  from mouse, rat and human samples.  Anti-CD73 (extracellular)-FITC Antibody (ABIN7042877, ABIN7044811 and ABIN7044812-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize NT5E from mouse, rat and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KGSTLKKAFEHSVHR, corresponding to amino acid residues 429-433 of mouse CD73

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582044)

Blocking Peptide: (ABIN7234599)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for CD73

Target: CD73 (NT5E) (5'-Nucleotidase, Ecto (CD73) (NT5E))

Alternative Name: NT5E

Background: Ecto-5-nucleotidase, E5NT, 5'-Nucleotidase, 5'-NT, NT5E, ENT,CD73 (ecto-5'-nucleotidase, NT5E) is a glycosylphosphatidylinositol enzyme expressed on the cell surface of various cell types. CD73 is responsible for hydrolyzing extracellular adenosine monophosphate to adenosine and inorganic phosphate, which results in activation of the G-protein coupled receptors, namely A2AR and A2BR, to exert potent immunoregulatory activity.CD73 plays an important role in homeostatic functions in healthy organs and tissues. The protein is involved as an inhibitory immune checkpoint molecule, it promotes immune escape of tumor cells since free adenosine generated by the protein inhibits cellular immune responses1,2.CD73 primarily detected in cancer cells and in immune cells such as CD4+Foxp3+ regulatory T cells, and myeloid-derived suppressor cells that are recruited by the tumor2.Studies show that CD73 expression is significantly up-regulated in numerous malignant tumors such as breast cancer, colorectal cancer, prostate cancer, ovarian cancer, and gallbladder cancer3.

Gene ID: 23959

UniProt: Q61503

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process