SLC2A3 antibody (Extracellular) (PE)

Catalog No. ABIN7582076

The Rabbit Polyclonal anti-SLC2A3 antibody is suitable to detect SLC2A3 in samples from Human. It has been validated for FACS and LCI.

Quick Overview for SLC2A3 antibody (Extracellular) (PE) (ABIN7582076)

Target: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLC2A3 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SLC2A3 Antibody (PE)

Binding Specificity: AA 39-51, Extracellular

Purpose: A Rabbit Polyclonal antibody to Human GLUT3 (extracellular) conjugated to R-Phycoerythrin (R-PE) fluorophore.

Sequence: (C)KEFINKTLTD KGN

Specificity: 1st extracellular loop

Cross-Reactivity (Details): It will not recognize rat or mouse GLUT3.

Predicted Reactivity: Human only This antibody will also recognize the human paralog GLUT14,SLC2A14 (Accession Q8TDB8) Homology - 12,13 amino acid residues identical The GLUT14 gene is specific to humans,has an overall 945% amino acid identity to GLUT3

Characteristics: Anti-Human GLUT3 (extracellular) Antibody (ABIN7043698, ABIN7044499 and ABIN7044500) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. The antibody has been designed to recognize GLUT3 from human samples only. This antibody will also recognize the human paralog GLUT14 /SLC2A14. Anti-Human GLUT3 (extracellular)-PE Antibody (ABIN7043698, ABIN7044499 and ABIN7044500-PE) is directly conjugated to R-Phycoerythrin (R-PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)KEFINKTLTDKGN, corresponding to amino acid residues 39 - 51 of human GLUT3

Isotype: IgG

Application Details

Application Notes: 1 μg peptide per 1 μg antibody

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide:

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 15 μL or 50 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SLC2A3

Target: SLC2A3 (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 3 (SLC2A3))

Alternative Name: SLC2A3

Background: Glucose transporter 3, Facilitated glucose transporter member 3, SLC2A3,The GLUT protein family is encoded by the SLC2 genes and is a member of the major facilitator superfamily of membrane transporters. GLUTs are recognized as key regulators of whole-body glucose homeostasis, and are responsible for the uptake of carbohydrate-derived glucose by cells. There are 14 GLUT isoforms divided into 3 different group classes based on sequence similarity and structural and functional characteristics1,2.GLUT3 is a low-Km protein responsible for glucose uptake into neurons. It is predominantly expressed in neurons and is commonly called "neuronal glucose transporter". The protein contains 12 membrane-spanning domains, an N-linked glycosylation site in loop 1 responsible for high-affinity transport of glucose, and intracellular NH2- and COOH- termini. In addition, there are several conserved residues and motifs designated "sugar transporter signatures"1,2.GLUT3 levels are high in some malignant glioma cells compared to normal glial cells and therefore may be used as a therapeutic target in some cancer treatment3.

Gene ID: 6515

UniProt: P11169

Pathways: Warburg Effect