SUCNR1 antibody (Extracellular) (PE)

Catalog No. ABIN7582080

This anti-SUCNR1 antibody is a Rabbit Polyclonal antibody detecting SUCNR1 in FACS and LCI. Suitable for Rat.

Quick Overview for SUCNR1 antibody (Extracellular) (PE) (ABIN7582080)

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SUCNR1 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Live Cell Imaging (LCI)

Product Details anti-SUCNR1 Antibody (PE)

Binding Specificity: AA 248-262, Extracellular

Purpose: A Rabbit Polyclonal Antibody to GPR91 (SUCNR1) (extracellular) conjugated to the fluorescent dye Phycoerythrin (PE)

Sequence: (C)RNVRIASRLD SWPQG

Specificity: 3rd extracellular loop

Predicted Reactivity: 15 amino acid residues identical,Mouse - 14, human - 12

Characteristics: Anti-GPR91 (SUCNR1) (extracellular) Antibody (ABIN7043783, ABIN7045308 and ABIN7045309) is a highly specific antibody directed against an extracellular epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and live cell imaging applications. It has been designed to recognize GPR91 from human, mouse and rat samples. Anti-GPR91 (SUCNR1) (extracellular)-PE Antibody (ABIN7043783, ABIN7045308 and ABIN7045309-PE) is directly conjugated to the Phycoerythrin (PE) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)RNVRIASRLDSWPQG, corresponding to amino acid residues 248-262 of rat GPR91/Succinate Receptor 1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: N/A

Comment:

Negative Control: (ABIN7582046)

Blocking Peptide: (ABIN7237300)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 50 μL double distilled water (DDW).

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for SUCNR1

Target: SUCNR1 (Succinate Receptor 1 (SUCNR1))

Alternative Name: SUCNR1

Background: G-protein coupled receptor 91, Succinate receptor 1,GPR91 (Succinate Receptor 1), a member of the GPCR superfamily, is specifically activated by extracellular succinate outside the tricarboxylic acid cycle, resulting in activation of paracrine and endocrine effectors. Activation of GPR91 is associated with several diseases, including hypertension and diabetes1,2.GPR91 was shown to regulate normal retinal vascularization, proliferative ischemic retinopathy and cortical revascularization post-ischemia. Activation of GPR91 influences different tissues such as kidney, heart, retina, white adipose tissue, and liver.Through the activation of GPR91, succinate affects motility, migration, and growth of dendritic cells. In addition, the receptor directly promotes chemotaxis and potentiates activation initiated by Toll-like receptor agonists in dendritic cells1-3.In the heart GPR91 mediates succinate-induced cardiomyocyte death. It was demonstrated that upholding levels of serum succinate can cause cardiac hypertrophy through the activation of the GPR911,2.

Gene ID: 408199

UniProt: Q6IYF9