GFRA1 antibody (AA 351-429)

Catalog No. ABIN726230

The Rabbit Polyclonal anti-GFRA1 antibody has been validated for WB, ELISA, FACS, IHC (p), IF (cc), IF (p) and IHC (fro). It is suitable to detect GFRA1 in samples from Human, Mouse and Goat. There is 1 publication available.

Quick Overview for GFRA1 antibody (AA 351-429) (ABIN726230)

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Reactivity: Human, Mouse, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFRA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-GFRA1 Antibody

Binding Specificity: AA 351-429

Specificity: This antibody exhibits a secondary cross-reactivity towards Exonuclease-1 due to a 60 % contigueous sequence similarity over the immunogen range.

Cross-Reactivity: Goat, Human, Mouse

Predicted Reactivity: Rat,Dog,Cow,Pig,Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human GFR Alpha-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-GFRA1 antibody (ABIN726230)

Ma, Zhou, Li, Zheng, Wu, Niu, Tang, He, Li, Hua: "Lin28a promotes self-renewal and proliferation of dairy goat spermatogonial stem cells (SSCs) through regulation of mTOR and PI3K/AKT." in: Scientific reports, Vol. 6, pp. 38805, (2016) (PubMed).

Target Details for GFRA1

Target: GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))

Alternative Name: GDNFRA

Background:

Synonyms: GDNFR, RET1L, RETL1, TRNR1, GDNFRA, GFR-ALPHA-1, GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, RET ligand 1, TGF-beta-related neurotrophic factor receptor 1, GFRA1

Background: Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor (By similarity).

Gene ID: 2674

UniProt: P56159