HCN1 antibody (AA 778-910) (FL650)

Catalog No. ABIN7595903

This Mouse Monoclonal antibody specifically detects HCN1 in IHC and ICC. It exhibits reactivity toward Rat.

Quick Overview for HCN1 antibody (AA 778-910) (FL650) (ABIN7595903)

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This HCN1 antibody is conjugated to FL650

Application: Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Clone: N70-28

Product Details anti-HCN1 Antibody (FL650)

Binding Specificity: AA 778-910

Purpose: Anti-HCN1 Antibody FL650 Conjugate

Specificity: No off-targets reported for HCN2

Cross-Reactivity: Gerbil, Human, Mouse, Non-Human Primate, Rat

Characteristics:

Description: Our Anti-HCN1 mouse monoclonal primary antibody is produced in-house from hybridoma clone N70/28. It is KO validated, detects human, mouse, and rat HCN1, and is purified by Protein A chromatography. It is great for use in IHC, ICC.

Manufacturer Comment: We produce our HCN1 mouse monoclonal primary antibody from hybridoma clone N70/28. It is great in IHC, ICC and is purified by Protein A chromatography.

Purification: Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb.

Purity: > 90 % specific antibody

Immunogen: Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (accession number Q9JKB0), Mouse: 96% identity (128/133 amino acids identical) produced recombinantly in E. Coli

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS with 0.09 % azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Expiry Date: 12 months

Target Details for HCN1

Target: HCN1 (Hyperpolarization Activated Cyclic Nucleotide-Gated Potassium Channel 1 (HCN1))

Alternative Name: HCN1

Background:

Synonyms: Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1

Target Description: HCN1 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN1 is found in the hippocampus, neocortex, and cerebellar cortex of brain. Alterations in this protein have been implicated in the disease early infantile epileptic encephalopathy.

Gene Name Alternatives: Hcn1

Molecular Weight: 100 kDa

Pathways: Asymmetric Protein Localization