USP25 antibody (AA 1-1053)

Catalog No. ABIN7598940

This Rabbit Polyclonal antibody specifically detects USP25 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human, Rat, Mouse and Monkey.

Quick Overview for USP25 antibody (AA 1-1053) (ABIN7598940)

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Reactivity: Human, Rat, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP25 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-USP25 Antibody

Binding Specificity: AA 1-1053

Purpose: Anti-USP25 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-USP25 Antibody Picoband® (ABIN7598940). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human USP25 recombinant protein (Position: M1-D1053).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat, Monkey
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Meulmeester, E., Kunze, M., Hsaio, H. H., Urlaub, H., Melchior, F. Mechanism and consequences for paralog-specific sumoylation of ubiquitin-specific protease 25. Molec. Cell 30: 610-619, 2008. 2. Valero, R., Bayes, M., Sanchez-Font, M. F., Gonzalez-Angulo, O., Gonzalez-Duarte, R., Marfany, G. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biol. 2: research0043, 2001. Note: Electronic Article. 3. Valero, R., Marfany, G., Gonzalez-Angulo, O., Gonzalez-Gonzalez, G., Puelles, L., Gonzales-Duarte, R. USP25, a novel gene encoding a deubiquitinating enzyme, is located in the gene-poor region 21q11.2. Genomics 62: 395-405, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for USP25

Target: USP25 (Ubiquitin Specific Peptidase 25 (USP25))

Alternative Name: USP25

Background:

Synonyms: Ubiquitin carboxyl-terminal hydrolase 25, Deubiquitinating enzyme 25, USP on chromosome 21, Ubiquitin thioesterase 25, Ubiquitin-specific-processing protease 25, USP25, USP21

Tissue Specificity: Isoform USP25a is found in most adult and fetal tissues, expression is moderately high in testis, pancreas, kidney, skeletal muscle, liver, lung, placenta, brain, heart, but very low in peripheral blood, colon, small intestine, ovary, prostate, thymus and spleen. Isoform USP25b is found in all tissues except heart and skeletal muscle. Isoform USP25m is heart and skeletal muscle specific.

Background: Ubiquitin specific peptidase 25 is a protein that in humans is encoded by the USP25 gene. It is mapped to 21q21.1. Ubiquitin is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains, which is mediated by ubiquitin-specific proteases (USPs), such as USP25.

Molecular Weight: 125 kDa

Gene ID: 29761