RPL27 antibody (AA 1-108)

Catalog No. ABIN7598946

This Rabbit Polyclonal antibody specifically detects RPL27 in WB, ELISA, IP and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for RPL27 antibody (AA 1-108) (ABIN7598946)

Target: RPL27 (Ribosomal Protein L27 (RPL27))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPL27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS)

Product Details anti-RPL27 Antibody

Binding Specificity: AA 1-108

Purpose: Anti-RPL27 Antibody Picoband®

Characteristics: Anti-RPL27 Antibody Picoband® (ABIN7598946). Tested in WB, IP, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RPL27 recombinant protein (Position: M1-R108). Human RPL27 shares 100% amino acid (aa) sequence identity with both mouse and rat RPL27.

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
1. Gallagher, R. A., McClean, P. M., Malik, A. N. Cloning and nucleotide sequence of a full length cDNA encoding ribosomal protein L27 from human fetal kidney. Biochim. Biophys. Acta 1217: 329-332, 1994. 2. Sim, E. U. H., Ang, C. H., Ng, C. C., Lee, C. W., Narayanan, K. Differential expression of a subset of ribosomal protein genes in cell lines derived from human nasopharyngeal epithelium. J. Hum. Genet. 55: 118-120, 2010. 3. Wang, R., Yoshida, K., Toki, T., Sawada, T., Uechi, T., Okuno, Y., Sato-Otsubo, A., Kudo, K., Kamimaki, I., Kanezaki, R., Shiraishi, Y., Chiba, K., and 21 others. Loss of function mutations in RPL27 and RPS27 identified by whole exome sequencing in Diamond-Blackfan anaemia. Brit. J. Haemat. 168: 854-864, 2015.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for RPL27

Target: RPL27 (Ribosomal Protein L27 (RPL27))

Alternative Name: RPL27

Background: 60S ribosomal protein L27 is a protein that in humans is encoded by the RPL27 gene. Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of four RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L27e family of ribosomal proteins and a component of the 60S subunit. A splice site mutation in this gene has been identified in a Diamond-Blackfan anemia (DBA) patient. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.

Molecular Weight: 16 kDa

Gene ID: 6155

UniProt: P61353