VAMP4 antibody (AA 1-118)

Catalog No. ABIN7598962

The Rabbit Polyclonal anti-VAMP4 antibody has been validated for WB, ELISA, IHC and FACS. It is suitable to detect VAMP4 in samples from Human, Mouse and Rat.

Quick Overview for VAMP4 antibody (AA 1-118) (ABIN7598962)

Target: VAMP4 (Vesicle-Associated Membrane Protein 4 (VAMP4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VAMP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-VAMP4 Antibody

Binding Specificity: AA 1-118

Purpose: Anti-VAMP4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-VAMP4 Antibody Picoband® (ABIN7598962). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human VAMP4 recombinant protein (Position: M1-A118).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Advani, R. J., Bae, H.-R., Bock, J. B., Chao, D. S., Doung, Y.-C., Prekeris, R., Yoo, J.-S., Scheller, R. H. Seven novel mammalian SNARE proteins localize to distinct membrane compartments. J. Biol. Chem. 273: 10317-10324, 1998. 2. Scott, A. F. Personal Communication. Baltimore, Md. 5/6/2002. 3. Steegmaier, M., Klumperman, J., Foletti, D. L., Yoo, J.-S., Scheller, R. H. Vesicle-associated membrane protein 4 is implicated in trans-Golgi network vesicle trafficking. Molec. Biol. Cell 10: 1957-1972, 1999.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for VAMP4

Target: VAMP4 (Vesicle-Associated Membrane Protein 4 (VAMP4))

Alternative Name: VAMP4

Background:

Synonyms: N-alpha-acetyltransferase 15, NatA auxiliary subunit,Gastric cancer antigen Ga19,N-terminal acetyltransferase,NMDA receptor-regulated protein 1,Protein tubedown-1,Tbdn100,NAA15,GA19, NARG1, NATH, TBDN100,

Tissue Specificity: Expressed at high levels in testis and in ocular endothelial cells. Also found in brain (corpus callosum), heart, colon, bone marrow and at lower levels in most adult tissues, including thyroid, liver, pancreas, mammary and salivary glands, lung, ovary, urogenital system and upper gastrointestinal tract. Overexpressed in gastric cancer, in papillary thyroid carcinomas and in a Burkitt lymphoma cell line (Daudi). Specifically suppressed in abnormal proliferating blood vessels in eyes of patients with proliferative diabetic retinopathy. .

Background: Vesicle-associated membrane protein 4 is a protein that in humans is encoded by the VAMP4 gene. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport.

Molecular Weight: 16 kDa

Gene ID: 8674

UniProt: O75379