HES5 antibody (AA 1-121)

Catalog No. ABIN7598966

This anti-HES5 antibody is a Rabbit Polyclonal antibody detecting HES5 in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for HES5 antibody (AA 1-121) (ABIN7598966)

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HES5 Antibody

Binding Specificity: AA 1-121

Purpose: Anti-HES5 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-HES5 Antibody Picoband® (ABIN7598966). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human HES5 recombinant protein (Position: M1-R121).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Katoh, M., Katoh, M. Identification and characterization of human HES2, HES3, and HES5 genes in silico. Int. J. Oncol. 25: 529-534, 2004. 2. Takebayashi, K., Akazawa, C., Nakanishi, S., Kageyama, R. Structure and promoter analysis of the gene encoding the mouse helix-loop-helix factor HES-5: identification of the neural precursor cell-specific promoter element. J. Biol. Chem. 270: 1342-1349, 1995.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for HES5

Target: HES5 (Hairy and Enhancer of Split 5 (HES5))

Alternative Name: HES5

Background:

Synonyms: Cytoskeleton-associated protein 5, Colonic and hepatic tumor overexpressed gene protein, Ch-TOG, CKAP5, KIAA0097

Tissue Specificity: Expressed in fetal brain. Highly expressed in brain and placenta. Lower levels in heart, liver, thymus, kidney and lung. Located to endothelial cells and neuronal cells of the suprachiasmatic nucleus (SCN). Also detected in endothelial cells of the heart, lung and kidney. In the brain, specifically expressed in the thalamus, hippocampus and amygdala.

Background: Transcription factor HES-5 is a protein that in humans is encoded by the HES5 gene. This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer.

Molecular Weight: 18 kDa

Gene ID: 388585

Pathways: Stem Cell Maintenance, Tube Formation