APAF1 antibody (AA 1-1248)

Catalog No. ABIN7598969

This anti-APAF1 antibody is a Rabbit Polyclonal antibody detecting APAF1 in WB, ELISA and FACS. Suitable for Human.

Quick Overview for APAF1 antibody (AA 1-1248) (ABIN7598969)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-APAF1 Antibody

Binding Specificity: AA 1-1248

Purpose: Anti-APAF1 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-APAF1 Antibody (ABIN7598969). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human APAF1 recombinant protein (Position: M1-E1248).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: APAF1

Background:

Synonyms: Zinc finger protein Gfi-1, Growth factor independent protein 1, Zinc finger protein 163, GFI1, ZNF163

Tissue Specificity: Isoform 1 is expressed in pancreas. Isoform 2 and isoform 3 is expressed in liver.

Background: Apoptotic peptidase activating factor 1, also known as APAF1, is a protein which in humans is encoded by the APAF1 gene. This gene is mapped to chromosome 12q23. It encodes a cytoplasmic protein that initiates apoptosis. And it is an essential downstream effector of p53-mediated apoptosis. This protein contains several copies of the WD40 repeat domain, a caspase recruitment domain(CARD), and an ATPase domain(NB-ARC). In the presence of cytochrome c and dATP, APAF1 assembles into an oligomeric apoptosome, which is responsible for activation of procaspase-9 and maintenance of the enzymatic activity of processed caspase-9. Furthermore, APAF1 is inactivated in metastatic melanomas, leading to defects in the execution of apoptotic cell death. Additionally, APAF1 has been shown to interact with NLRP1, Caspase-9, APIP, BCL2-like 1 and HSPA4.

Molecular Weight: 135 kDa

Gene ID: 317

UniProt: O14727

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity