SIVA1 antibody (AA 1-141)

Catalog No. ABIN7598994

This Rabbit Polyclonal antibody specifically detects SIVA1 in WB, ELISA and FACS. It exhibits reactivity toward Human.

Quick Overview for SIVA1 antibody (AA 1-141) (ABIN7598994)

Target: SIVA1 (SIVA1, Apoptosis-Inducing Factor (SIVA1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIVA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-SIVA1 Antibody

Binding Specificity: AA 1-141

Purpose: Anti-SIVA/SIVA1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-SIVA/SIVA1 Antibody Picoband® (ABIN7598994). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human SIVA/SIVA1 recombinant protein (Position: M1-T141).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Nestler, M., Martin, U., Hortschansky, P., Saluz, H.-P., Henke, A., Munder, T. The zinc containing pro-apoptotic protein siva interacts with the peroxisomal membrane protein pmp22. Molec. Cell. Biochem. 287: 147-155, 2006. 2. Prasad, K. V. S., Ao, Z., Yoon, Y., Wu, M. X., Rizk, M., Jacquot, S., Schlossman, S. F. CD27, a member of the tumor necrosis factor receptor family, induces apoptosis and binds to Siva, a proapoptotic protein. Proc. Nat. Acad. Sci. 94: 6346-6351, 1997. 3. Py, B., Slomianny, C., Auberger, P., Petit, P. X., Benichou, S. Siva-1 and an alternative splice form lacking the death domain, Siva-2, similarly induce apoptosis in T lymphocytes via a caspase-dependent mitochondrial pathway. J. Immun. 172: 4008-4017, 2004.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for SIVA1

Target: SIVA1 (SIVA1, Apoptosis-Inducing Factor (SIVA1))

Alternative Name: SIVA1

Background:

Synonyms: POU domain, class 4, transcription factor 1

Tissue Specificity: Expressed in the brain and the retina. Present in the developing brain, spinal cord and eye.

Background: Apoptosis regulatory protein Siva is a protein that in humans is encoded by the SIVA1 gene. This gene encodes an E3 ubiquitin ligase that regulates cell cycle progression, cell proliferation and apoptosis. The N-terminus of this protein binds to the cytoplasmic tail of the CD27 antigen, a member of the tumor necrosis factor receptor (TNFR) superfamily. In response to UV radiation-induced DNA damage, this protein has been shown to mediate the ubiquitination of proliferating cell nuclear antigen (PCNA), an important step in translesion DNA synthesis.

Molecular Weight: 19 kDa

Gene ID: 10572

UniProt: O15304

Pathways: Positive Regulation of Endopeptidase Activity