UBD antibody (AA 1-163)

Catalog No. ABIN7599019

The Rabbit Polyclonal anti-UBD antibody has been validated for WB and ELISA. It is suitable to detect UBD in samples from Human.

Quick Overview for UBD antibody (AA 1-163) (ABIN7599019)

Target: UBD (Ubiquitin D (UBD))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UBD antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-UBD Antibody

Binding Specificity: AA 1-163

Purpose: Anti-FAT10/UBD Picoband® Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-FAT10/UBD Picoband® Antibody (ABIN7599019). Tested in ELISA, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human FAT10/UBD recombinant protein (Position: M1-I163).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL, -
1. Bates, E. E. M., Ravel, O., Dieu, M.-C., Ho, S., Guret, C., Bridon, J.-M., Ait-Yahia, S., Briere, F., Caux, C., Banchereau, J., Lebecque, S. Identification and analysis of a novel member of the ubiquitin family expressed in dendritic cells and mature B cells. Europ. J. Immun. 27: 2471-2477, 1997. 2. Buchsbaum, S., Bercovich, B., Ciechanover, A. FAT10 is a proteasomal degradation signal that is itself regulated by ubiquitination. Molec. Biol. Cell 23: 225-232, 2012. 3. Chiu, Y.-H., Sun, Q., Chen, Z. J. E1-L2 activates both ubiquitin and FAT10. Molec. Cell 27: 1014-1023, 2007.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for UBD

Target: UBD (Ubiquitin D (UBD))

Alternative Name: UBD

Background:

Synonyms: Ubiquitin D, Diubiquitin, Ubiquitin-like protein FAT10, UBD, FAT10

Tissue Specificity: Preferentially expressed in activated T lymphocytes.

Background: Ubiquitin D is a protein that in humans is encoded by the UBD gene. It is mapped to 6p22.1. This gene encodes a protein which contains two ubiquitin-like domains and appears to have similar function to ubiquitin. Through covalent attachment, the encoded protein targets other proteins for 26S proteasome degradation. This protein has been implicated to function in many cellular processes, including caspase-dependent apoptosis, formation of aggresomes, mitotic regulation, and dendritic cell maturation. Upregulation of this gene may promote inflammation in chronic kidney disease and has been observed in many cancer types.

Molecular Weight: 18 kDa

Gene ID: 10537

UniProt: O15205

Pathways: Ubiquitin Proteasome Pathway