ABCB5 antibody (AA 1-176)

Catalog No. ABIN7599033

The Rabbit Polyclonal anti-ABCB5 antibody (ABIN7599033) specifically detects ABCB5 in WB, ELISA, IHC, FACS, IF and ICC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ABCB5 antibody (AA 1-176) (ABIN7599033)

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ABCB5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-ABCB5 Antibody

Binding Specificity: AA 1-176

Purpose: Anti-ABCB5 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ABCB5 Antibody (ABIN7599033). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ABCB5 recombinant protein (Position: M1-D176).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ABCB5

Target: ABCB5 (ATP-Binding Cassette, Sub-Family B (MDR/TAP), Member 5 (ABCB5))

Alternative Name: ABCB5

Background:

Synonyms: ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5

Tissue Specificity: Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.

Background: ATP-binding cassette sub-family B member 5 also known as P-glycoprotein ABCB5 is a plasma membrane-spanning protein that in humans is encoded by the ABCB5 gene.It is mapped to 7p21.1. ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

Molecular Weight: 110 kDa

Gene ID: 340273

UniProt: Q2M3G0