NSG1 antibody (AA 1-176)

Catalog No. ABIN7599034

This Rabbit Polyclonal antibody specifically detects NSG1 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for NSG1 antibody (AA 1-176) (ABIN7599034)

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NSG1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-NSG1 Antibody

Binding Specificity: AA 1-176

Purpose: Anti-NSG1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-NSG1 Antibody Picoband® (ABIN7599034). Tested in ELISA, IHC, WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human NSG1 recombinant protein (Position: M1-Q176).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Carlock, L., Vo, T., Lorincz, M., Walker, P. D., Bessert, D., Wisniewski, D., Dunbar, J. C. Variable subcellular localization of a neuron-specific protein during NTera 2 differentiation into post-mitotic human neurons. Molec. Brain Res. 42: 202-212, 1996. 2. Carlock, L., Wisniewski, D., Lorincz, M., Pandrangi, A., Vo, T. An estimate of the number of genes in the Huntington disease gene region and the identification of 13 transcripts in the 4p16.3 segment. Genomics 13: 1108-1118, 1992.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for NSG1

Target: NSG1 (D4S234E) (Neuron-Specific Protein Family Member 1 (D4S234E))

Alternative Name: NSG1

Background:

Synonyms: Negative elongation factor B, NELF-B, Cofactor of BRCA1, NELFB, COBRA1, KIAA1182

Tissue Specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.

Background: NSG1, also known as Neuron-specific protein family member 1, contains an N-terminal nuclear localization motif and a central hydrophobic segment. NSG1 localizes to the nucleus in mitotic neuronal cell cultures and to the cytoplasm in postmitotic neurons. Mouse and human NSG1 share 97 % sequence identity and they differs from each other by only 5 amino acid residues. The protein is expressed in all regions of rat brain. In hamster, it was preferentially expressed in adult brain.GenScript Rabbit Anti-NSG1 Polyclonal Antibody is developed in rabbit using purified His-tagged human NSG1 full length recombinant protein.

Molecular Weight: 21 kDa

Gene ID: 27065

UniProt: P42857