PPIH antibody (AA 1-177)

Catalog No. ABIN7599038

Product Details anti-PPIH Antibody

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Binding Specificity: AA 1-177

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PPIH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Purpose: Anti-PPIH Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-PPIH Antibody Picoband® (ABIN7599038). Tested in WB, ICC/IF, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PPIH recombinant protein (Position: M1-M177). Human PPIH shares 100% amino acid (aa) sequence identity with mouse PPIH.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL
1. Horowitz, D. S., Kobayashi, R., Krainer, A. R. A new cyclophilin and the human homologues of yeast Prp3 and Prp4 form a complex associated with U4/U6 snRNPs. RNA 3: 1374-1387, 1997. 2. Reidt, U., Reuter, K., Achsel, T., Ingelfinger, D., Luhrmann, R., Ficner, R. Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin. J. Biol. Chem. 275: 7439-7442, 2000. 3. Teigelkamp, S., Achsel, T., Mundt, C., Gothel, S.-F., Cronshagen, U., Lane, W. S., Marahiel, M., Luhrmann, R. The 20kD protein of human [U4/U6.U5] tri-snRNPs is a novel cyclophilin that forms a complex with the U4/U6-specific 60kD and 90kD proteins. RNA 4: 127-141, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PPIH

Target: PPIH (Peptidylprolyl Isomerase H (Cyclophilin H) (PPIH))

Alternative Name: PPIH (PPIH Products)

Background:

Synonyms: PPIH, CYP20, CYPH, Peptidyl-prolyl cis-trans isomerase H, PPIase H, EC 5.2.1.8, Rotamase H, Small nuclear ribonucleoprotein particle-specific cyclophilin H, Cy pH , U-snRNP-associated cyclophilin SnuCyp-20, USA-CYP

Background: The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is a specific component of the complex that includes pre-mRNA processing factors PRPF3, PRPF4, and PRPF18, as well as U4/U5/U6 tri-snRNP. This protein has been shown to possess PPIase activity and may act as a protein chaperone that mediates the interactions between different proteins inside the spliceosome.

Molecular Weight: 19 kDa

Gene ID: 10465

UniProt: O43447