PDXP antibody (AA 1-189)

Catalog No. ABIN7599053

The Rabbit Polyclonal anti-PDXP antibody has been validated for ELISA, WB and FACS. It is suitable to detect PDXP in samples from Mouse, Human and Rat.

Quick Overview for PDXP antibody (AA 1-189) (ABIN7599053)

Target: PDXP (Pyridoxal (Pyridoxine, Vitamin B6) Phosphatase (PDXP))

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PDXP antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-PDXP Antibody

Binding Specificity: AA 1-189

Purpose: Anti-PDXP Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-PDXP Antibody Picoband® (ABIN7599053). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human PDXP recombinant protein (Position: M1-R189).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Gohla, A., Birkenfeld, J., Bokoch, G. M. Chronophin, a novel HAD-type serine protein phosphatase, regulates cofilin-dependent actin dynamics. Nature Cell Biol. 7: 21-29, 2005. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 02/27/2017. 3. Huang, T. Y., Michael, S., Xu, T., Sarkeshik, A., Moresco, J. J., Yates, J. R., III, Masliah, E., Bokoch, G. M., DerMardirossian, C. A novel Rac1 GAP splice variant relays poly-Ub accumulation signals to mediate Rac1 inactivation. Molec. Biol. Cell 24: 194-209, 2013.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for PDXP

Target: PDXP (Pyridoxal (Pyridoxine, Vitamin B6) Phosphatase (PDXP))

Alternative Name: PDXP

Background:

Synonyms: Peflin, PEF protein with a long N-terminal hydrophobic domain, Penta-EF hand domain-containing protein 1, PEF1, ABP32, UNQ1845, PRO3573

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: Pyridoxal 5-prime-phosphate (PLP) is the active form of vitamin B6 that acts as a coenzyme in maintaining biochemical homeostasis. The preferred degradation route from PLP to 4-pyridoxic acid involves the dephosphorylation of PLP by PDXP.

Molecular Weight: 37 kDa

Gene ID: 57026