DOCK8 antibody (AA 1-2099)

Catalog No. ABIN7599085

Product Details anti-DOCK8 Antibody

Target: DOCK8 (Dedicator of Cytokinesis 8 (DOCK8))

Binding Specificity: AA 1-2099

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DOCK8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Purpose: Anti-DOCK8 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-DOCK8 Antibody Picoband® (ABIN7599085). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human DOCK8 recombinant protein (Position: M1-S2099). Human DOCK8 shares 91.8% amino acid (aa) sequence identity with mouse DOCK8.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL
1. Griggs, B. L., Ladd, S., Saul, R. A., DuPont, B. R., Srivastava, A. K. Dedicator of cytokinesis 8 is disrupted in two patients with mental retardation and developmental disabilities. Genomics 91: 195-202, 2008. 2. MacDermot, K. D., Hulten, M. Female with hypohidrotic ectodermal dysplasia and de novo (X,9) translocation: clinical documentation of the AnLy cell line case. Hum. Genet. 84: 577-579, 1990. 3. Pillay, B. A., Fusaro, M., Gray, P. E., Statham, A. L., Burnett, L., Bezrodnik, L., Kane, A., Tong, W., Abdo, C., Winter, S., Chevalier, S., Levy, R., and 18 others. Somatic reversion of pathogenic DOCK8 variants alters lymphocyte differentiation and function to effectively cure DOCK8 deficiency. J. Clin. Invest. 131: e142434, 2021.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for DOCK8

Target: DOCK8 (Dedicator of Cytokinesis 8 (DOCK8))

Alternative Name: DOCK8 (DOCK8 Products)

Background:

Synonyms: DOCK8, Dedicator of cytokinesis protein 8

Background: This gene encodes a member of the DOCK180 family of guanine nucleotide exchange factors. Guanine nucleotide exchange factors interact with Rho GTPases and are components of intracellular signaling networks. Mutations in this gene result in the autosomal recessive form of the hyper-IgE syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 230 kDa

Gene ID: 81704