BRD2 antibody (AA 1-214)

Catalog No. ABIN7599091

This Rabbit Polyclonal antibody specifically detects BRD2 in WB, ELISA, ICC, IF and FACS. It exhibits reactivity toward Human.

Quick Overview for BRD2 antibody (AA 1-214) (ABIN7599091)

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Product Details anti-BRD2 Antibody

Binding Specificity: AA 1-214

Purpose: Anti-BRD2 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-BRD2 Antibody Picoband® (ABIN7599091). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human BRD2 recombinant protein (Position: M1-Q214).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Beck, S., Hanson, I., Kelly, A., Pappin, D. J., Trowsdale, J. A homologue of the Drosophila female sterile homeotic (fsh) gene in the class II region of the human MHC. DNA Seq. 2: 203-210, 1992. 2. Dawson, M. A., Prinjha, R. K., Dittmann, A., Giotopoulos, G., Bantscheff, M., Chan, W.-I., Robson, S. C., Chung, C., Hopf, C., Savitski, M. M., Huthmacher, C., Gudgin, E., and 15 others. Inhibition of BET recruitment to chromatin as an effective treatment for MLL-fusion leukaemia. Nature 478: 529-533, 2011. 3. Denis, G. V., Green, M. R. A novel, mitogen-activated nuclear kinase is related to a Drosophila developmental regulator. Genes Dev. 10: 261-271, 1996.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for BRD2

Target: BRD2 (Bromodomain Containing 2 (BRD2))

Alternative Name: BRD2

Background:

Synonyms: Bromodomain-containing protein 2, O27.1.1, Really interesting new gene 3 protein, BRD2, KIAA9001, RING3

Tissue Specificity: Expressed in thymus, spleen, bone marrow, kidney, brain and testis, but hardly in all other tissues or in whole embryos during development.

Background: Bromodomain-containing protein 2 is a protein that in humans is encoded by the BRD2 gene. It is mapped to 6p21.32. This gene encodes a transcriptional regulator that belongs to the BET (bromodomains and extra terminal domain) family of proteins. This protein associates with transcription complexes and with acetylated chromatin during mitosis, and it selectively binds to the acetylated lysine-12 residue of histone H4 via its two bromodomains. The gene maps to the major histocompatability complex (MHC) class II region on chromosome 6p21.3, but sequence comparison suggests that the protein is not involved in the immune response. This gene has been implicated in juvenile myoclonic epilepsy, a common form of epilepsy that becomes apparent in adolescence. Multiple alternatively spliced variants have been described for this gene.

Molecular Weight: 110 kDa

Gene ID: 6046

UniProt: P25440

Pathways: Chromatin Binding, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection