TRIM16 antibody (AA 1-218)

Catalog No. ABIN7599098

The Rabbit Polyclonal anti-TRIM16 antibody has been validated for ELISA, WB, IHC and FACS. It is suitable to detect TRIM16 in samples from Human and Rat.

Quick Overview for TRIM16 antibody (AA 1-218) (ABIN7599098)

Target: TRIM16 (Tripartite Motif Containing 16 (TRIM16))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRIM16 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-TRIM16 Antibody

Binding Specificity: AA 1-218

Purpose: Anti-TRIM16 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-TRIM16 Antibody Picoband® (ABIN7599098). Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human TRIM16 recombinant protein (Position: M1-Q218).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.25 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Beer, H.-D., Munding, C., Dubois, N., Mamie, C., Hohl, D., Werner, S. The estrogen-responsive B box protein: a novel regulator of keratinocyte differentiation. J. Biol. Chem. 277: 20740-20749, 2002. 2. Liu, H.-L. C., Golder-Novoselsky, E., Seto, M. H., Webster, L., McClary, J., Zajchowski, D. A. The novel estrogen-responsive B box protein (EBBP) gene is tamoxifen regulated in cells expressing an estrogen receptor DNA-binding domain mutant. Molec. Endocr. 12: 1733-1748, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for TRIM16

Target: TRIM16 (Tripartite Motif Containing 16 (TRIM16))

Alternative Name: TRIM16

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP, MED20, TRFP,

Tissue Specificity: Pre-B-cells and B-cells but not terminally differentiated plasma cells.

Background: Tripartite motif-containing protein 16 is a protein that in humans is encoded by the TRIM16 gene. The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy.

Molecular Weight: 64 kDa

Gene ID: 10626

UniProt: O95361

Pathways: Retinoic Acid Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Growth Factor Binding