CKAP5 antibody (AA 1-221)

Catalog No. ABIN7599106

This Mouse Monoclonal antibody specifically detects CKAP5 in WB, IF, IHC, FACS and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CKAP5 antibody (AA 1-221) (ABIN7599106)

Target: CKAP5 (Cytoskeleton Associated Protein 5 (CKAP5))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CKAP5 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 3C13

Product Details anti-CKAP5 Antibody

Binding Specificity: AA 1-221

Purpose: Anti-ch TOG/CKAP5 Antibody Picoband® (monoclonal, 3C13)

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ch TOG/CKAP5 Antibody Picoband® (monoclonal, 3C13) (ABIN7599106). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human ch TOG/CKAP5 recombinant protein (Position: M1-L221).

Isotype: IgG2b

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1."Entrez Gene: CKAP5 cytoskeleton associated protein 5". 2.Charrasse S, Mazel M, Taviaux S, Berta P, Chow T, Larroque C (Feb 1996). "Characterization of the cDNA and pattern of expression of a new gene over-expressed in human hepatomas and colonic tumors". Eur. J. Biochem. 234 (2): 406-13.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CKAP5

Target: CKAP5 (Cytoskeleton Associated Protein 5 (CKAP5))

Alternative Name: CKAP5

Background:

Synonyms: 3-oxo-5-beta-steroid 4-dehydrogenase, Aldo-keto reductase family 1 member D1, Delta (4)-3-ketosteroid 5-beta-reductase, Delta (4)-3-oxosteroid 5-beta-reductase, AKR1D1, SRD5B1

Tissue Specificity: Highly expressed in liver. Expressed in testis and weakly in colon.

Background: Cytoskeleton-associated protein 5 is a microtubule-associated protein that in humans is encoded by the CKAP5 gene. It is mapped to 11p11.2. This gene encodes a cytoskeleton-associated protein which belongs to the TOG/XMAP215 family. The N-terminal half of this protein contains a microtubule-binding domain and the C-terminal half contains a KXGS motif for binding tubulin dimers. This protein has two distinct roles in spindle formation, it protects kinetochore microtubules from depolymerization and plays an essential role in centrosomal microtubule assembly. This protein may be necessary for the proper interaction of microtubules with the cell cortex for ional cell movement. It also plays a role in translation of the myelin basic protein (MBP) mRNA by interact ernatively spliced transcript variants encoding different isoforms have been identified.

Molecular Weight: 225 kDa

Gene ID: 9793

UniProt: Q14008

Pathways: M Phase