RIOK1 antibody (AA 1-222)

Catalog No. ABIN7599108

This anti-RIOK1 antibody is a Rabbit Polyclonal antibody detecting RIOK1 in WB, ELISA and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for RIOK1 antibody (AA 1-222) (ABIN7599108)

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RIOK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Product Details anti-RIOK1 Antibody

Binding Specificity: AA 1-222

Purpose: Anti-RIOK1/RIO1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RIOK1/RIO1 Antibody Picoband® (ABIN7599108). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RIOK1/RIO1 recombinant protein (Position: M1-K222).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Ameismeier, M., Cheng, J., Berninghausen, O., Beckmann, R. Visualizing late states of human 40S ribosomal subunit maturation. Nature 558: 249-253, 2018. 2. Guderian, G., Peter, C., Wiesner, J., Sickmann, A., Schulze-Osthoff, K., Fischer, U., Grimmler, M. RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity. J. Biol. Chem. 286: 1976-1986, 2011 3. Hartz, P. A. Personal Communication. Baltimore, Md. 10/31/2017.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RIOK1

Target: RIOK1 (RIO Kinase 1 (RIOK1))

Alternative Name: RIOK1

Background:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Serine/threonine-protein kinase RIO1 is an enzyme that in humans is encode by the RIOK1 gene. The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits.

Molecular Weight: 66 kDa

Gene ID: 83732