RGS18 antibody (AA 1-230)

Catalog No. ABIN7599123

Product Details anti-RGS18 Antibody

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Binding Specificity: AA 1-230

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RGS18 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Purpose: Anti-RGS18 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-RGS18 Antibody Picoband® (ABIN7599123). Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human RGS18 recombinant protein (Position: M1-D230).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Park, I.-K., Klug, C. A., Li, K., Jerabek, L., Li, L., Nanamori, M., Neubig, R. R., Hood, L., Weissman, I. L., Clarke, M. F. Molecular cloning and characterization of a novel regulator of G-protein signaling from mouse hematopoietic stem cells. J. Biol. Chem. 276: 915-923, 2001. 2. Sierra, D. A., Gilbert, D. J., Householder, D., Grishin, N. V., Yu, K., Ukidwe, P., Barker, S. A., He, W., Wensel, T. G., Otero, G., Brown, G., Copeland, N. G., Jenkins, N. A., Wilkie, T. M. Evolution of the regulators of G-protein signaling multigene family in mouse and human. Genomics 79: 177-185, 2002. 3.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for RGS18

Target: RGS18 (Regulator of G-Protein Signalling 18 (RGS18))

Alternative Name: RGS18 (RGS18 Products)

Background:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Regulator of G-protein signaling 18 is a protein that in humans is encoded by the RGS18 gene. This gene encodes a member of the regulator of G-protein signaling family. This protein is contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Molecular Weight: 27 kDa

Gene ID: 64407

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling