C21ORF45 antibody (AA 1-233)

Catalog No. ABIN7599127

This Rabbit Polyclonal antibody specifically detects C21ORF45 in WB, ELISA, IF, FACS and ICC. It exhibits reactivity toward Human.

Quick Overview for C21ORF45 antibody (AA 1-233) (ABIN7599127)

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C21ORF45 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Product Details anti-C21ORF45 Antibody

Binding Specificity: AA 1-233

Purpose: Anti-C21orf45/MIS18A Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-C21orf45/MIS18A Antibody Picoband® (ABIN7599127). Tested in ELISA, IF, ICC, WB, Flow Cytometry applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human C21orf45/MIS18A recombinant protein (Position: M1-D233).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C., Yanagida, M. Priming of centromere for CENP-A recruitment by human hMi18-alpha, hMis18-beta, and M18BP1. Dev. Cell 12: 17-30, 2007. 2. Gross, M. B. Personal Communication. Baltimore, Md. 9/28/2018. 3. Kim, I. S., Lee, M., Park, K. C., Jeon, Y., Park, J. H., Hwang, E. J., Jeon, T. I., Ko, S., Lee, H., Baek, S. H., Kim, K. I. Roles of Mis18-alpha in epigenetic regulation of centromeric chromatin and CENP-A loading. Molec. Cell 46: 260-273, 2012.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for C21ORF45

Target: C21ORF45 (MIS18A) (MIS18 Kinetochore Protein Homolog A (MIS18A))

Alternative Name: MIS18A

Background:

Synonyms: RNA-binding protein Nova-2, Astrocytic NOVA1-like RNA-binding protein, Neuro-oncological ventral antigen 2, NOVA2, ANOVA, NOVA3

Tissue Specificity: Brain. Expression restricted to astrocytes.

Background: Protein Mis18-alpha is a protein that in humans is encoded by the MIS18A gene. C21orf45, also named as MIS18A, C21orf46 and FASP1, is required for recruitment of CENPA to centromeres and normal chromosome segregation during mitosis. C21orf45 has dimer (~52 kDa) and heterodimer(~105 kDa) forms.

Molecular Weight: 26 kDa

Gene ID: 54069