CLASP1 antibody (AA 1-243)

Catalog No. ABIN7599144

This anti-CLASP1 antibody is a Rabbit Polyclonal antibody detecting CLASP1 in WB, IHC, ELISA and IF. Suitable for Human and Rat.

Quick Overview for CLASP1 antibody (AA 1-243) (ABIN7599144)

Target: CLASP1 (Cytoplasmic Linker Associated Protein 1 (CLASP1))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CLASP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CLASP1 Antibody

Binding Specificity: AA 1-243

Purpose: Anti-CLASP1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-CLASP1 Antibody Picoband® (ABIN7599144). Tested in ELISA, IF, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human CLASP1 recombinant protein (Position: M1-D243).

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL/mL, Human, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL/mL, Human, Rat
Immunofluorescence, 5 μg/mL, Human
ELISA, 0.1-0.5 μg/mL/mL, Human
1. Akhmanova, A., Hoogenraad, C. C., Drabek, K., Stepanova, T., Dortland, B., Verkerk, T., Vermeulen, W., Burgering, B. M., De Zeeuw, C. I., Grosveld, F., Galjart, N. CLASPs are CLIP-115 and -170 associating proteins involved in the regional regulation of microtubule dynamics in motile fibroblasts. Cell 104: 923-935, 2001. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 10/31/2013. 3. Ishikawa, K., Nagase, T., Suyama, M., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. X. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro. DNA Res. 5: 169-176, 1998.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for CLASP1

Target: CLASP1 (Cytoplasmic Linker Associated Protein 1 (CLASP1))

Alternative Name: CLASP1

Background:

Synonyms: Mediator of RNA polymerase II transcription subunit 14, Activator-recruited cofactor 150 kDa component, ARC150, Cofactor required for Sp1 transcriptional activation subunit 2, CRSP complex subunit 2, Mediator complex subunit 14, RGR1 homolog, hRGR1, Thyroid hormone receptor-associated protein complex 170 kDa component, Trap170, Transcriptional coactivator CRSP150, Vitamin D3 receptor-interacting protein complex 150 kDa component, DRIP150, MED14, ARC150, CRSP2, CXorf4, DRIP150, EXLM1, RGR1, TRAP170,

Tissue Specificity: Ubiquitous.

Background: Cytoplasmic linker associated protein 1, also known as CLASP1, is a protein which in humans is encoded by the CLASP1 gene. CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170, MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle.

Molecular Weight: 150 kDa

Gene ID: 23332

UniProt: Q7Z460

Pathways: Microtubule Dynamics, M Phase, Maintenance of Protein Location