MIOX antibody (AA 1-266)

Catalog No. ABIN7599187

This Rabbit Polyclonal antibody specifically detects MIOX in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for MIOX antibody (AA 1-266) (ABIN7599187)

Target: MIOX (Myo-Inositol Oxygenase (MIOX))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIOX antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-MIOX Antibody

Binding Specificity: AA 1-266

Purpose: Anti-MIOX Antibody

Characteristics: Anti-MIOX Antibody (ABIN7599187). Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MIOX recombinant protein (Position: M1-K266). Human MIOX shares 89.8% and 90.5% amino acid (aa) sequence identity with mouse and rat MIOX, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.25-0.5 μg/mL, Human, Mouse
Immunohistochemistry, 2-5 μg/mL, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Storage: 4 °C,-20 °C

Storage Comment: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Target Details for MIOX

Target: MIOX (Myo-Inositol Oxygenase (MIOX))

Alternative Name: MIOX

Background: Myo-inositol oxygenase (MIOX), also known as ALDRL6, is a renal-specific member of the Aldo-keto reductase family. It catalyzes the first committed step in the Myo-inositol metabolism pathway and is widely distributed in mammalian tissues. Human Myo-inositol oxygenase shares 91 % and 96 % sequence homology with mouse and pig Myo-inositol oxygenase homologs, respectively. Myo-inositol oxygenase is responsible for the oxidative cleavage of Myo-inositol (MI) and its epimer D-chiro inositol (DCI) to D-glucuronate. The dioxygen-dependent cleavage of the C1-C6 bond in Myo-inositol is accomplished through the utilization of the FeII/FeIII binuclear iron center of MIOX. Myo-inositol oxygenase has also been implicated in complications of diabetes, including diabetic nephropathy.

Molecular Weight: 36 kDa

Gene ID: 55586

Pathways: Inositol Metabolic Process