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NOSIP antibody (AA 1-278)

The Rabbit Polyclonal anti-NOSIP antibody has been validated for WB, ELISA, IF, ICC and FACS. It is suitable to detect NOSIP in samples from Human.
Catalog No. ABIN7599206

Quick Overview for NOSIP antibody (AA 1-278) (ABIN7599206)

Target

See all NOSIP Antibodies
NOSIP (Nitric Oxide Synthase Interacting Protein (NOSIP))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This NOSIP antibody is un-conjugated

Application

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Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)
  • Binding Specificity

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    AA 1-278

    Purpose

    Anti-NOSIP Antibody Picoband®

    Cross-Reactivity (Details)

    No cross-reactivity with other proteins.

    Characteristics

    Anti-NOSIP Antibody Picoband® (ABIN7599206). Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Purification

    Immunogen affinity purified.

    Immunogen

    E.coli-derived human NOSIP recombinant protein (Position: M1-R278).

    Isotype

    IgG
  • Application Notes

    Western blot, 0.25-0.5 μg/mL, Human
    Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
    Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
    ELISA, 0.1-0.5 μg/mL, -
    1. Dedio, J., Konig, P., Wohlfart, P., Schroeder, C., Kummer, W., Muller-Esterl, W. NOSIP, a novel modulator of endothelial nitric oxide synthase activity. FASEB J. 15: 79-89, 2001. 2. Hoffmeister, M., Prelle, C., Kuchler, P., Kovacevic, I., Moser, M., Muller-Esterl, W., Oess, S. The ubiquitin E3 ligase NOSIP modulates protein phosphatase 2A activity in craniofacial development. PLoS One 9: e116150, 2014. Note: Electronic Article.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Concentration

    500 μg/mL

    Buffer

    Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

    Storage

    4 °C,-20 °C

    Storage Comment

    At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
  • Target

    NOSIP (Nitric Oxide Synthase Interacting Protein (NOSIP))

    Alternative Name

    NOSIP

    Background

    Synonyms: C-C motif chemokine 16, Chemokine CC-4, HCC-4, Chemokine LEC, IL-10-inducible chemokine, LCC-1, Liver-expressed chemokine, Lymphocyte and monocyte chemoattractant, LMC, Monotactin-1, MTN-1, NCC-4, Small-inducible cytokine A16, CCL16, ILINCK, NCC4, SCYA16

    Tissue Specificity: Mainly expressed in liver, also found in spleen and thymus. Highly expressed in LPS- and IFN-gamma- activated monocytes, weakly in some lymphocytes, including natural killer cells, gamma-delta T-cells, and some T-cell clones.

    Background: Nitric oxide synthase-interacting protein is an enzyme that in humans is encoded by the NOSIP gene. The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein.

    Molecular Weight

    35 kDa

    Gene ID

    51070

    UniProt

    Q9Y314
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